Detection of urinary excreted fungal galactomannan-like antigens for diagnosis of invasive aspergillosis.
Detection of urinary excreted fungal galactomannan-like antigens for diagnosis of invasive aspergillosis.
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DOI：
10.1371/journal.pone.0042736
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Marr KA
中科院分区：
文献类型：
--
作者：
Dufresne SF;Datta K;Li X;Dadachova E;Staab JF;Patterson TF;Feldmesser M;Marr KA
与侵袭性曲霉病(IA)相关的死亡率仍然很高，部分原因是诊断延迟。检测感染期间在血清和其他体液中释放的微生物外源抗原可能有助于及时诊断。在IA的过程中，半乳甘露聚糖(AspergillusGalactomanan，GM)是一种公认的多糖生物标志物，在包括尿液在内的体液中释放。尿液是一种丰富的、安全收集的样本，非常适合于护理点(POC)测试，这将在早期疾病筛查中发挥越来越大的作用。我们的主要目标是证明GM抗原性尿是一种临床上与IA相关的生物学现象，并建立概念证明，即它可以转化为POC诊断。利用一种识别曲霉和其他霉菌中的GM样抗原的新型IgM单抗(MAb476)，我们在实验性动物IA模型(豚鼠)以及人类患者中证明了抗原性尿。此外，我们还调查了人体样本中尿液排泄抗原的化学性质，用免疫分析方法表征了尿液中抗原的检测，描述了尿液中一种可能的检测抑制物，并指出了减轻抑制作用的方法。我们还设计并使用了一种侧向流动免疫层析方法来检测有限数量的IA患者尿样中的尿液排泄抗原。在本研究中，我们建立了基于尿粒细胞巨噬细胞集落刺激因子检测的脑出血POC诊断是可行的。有必要进行前瞻性研究，以确定优化设备的性能特征，并确定其最佳临床应用。
Mortality associated with invasive aspergillosis (IA) remains high, partly because of delayed diagnosis. Detection of microbial exoantigens, released in serum and other body fluids during infection, may help timely diagnosis. In course of IA, Aspergillus galactomannan (GM), a well established polysaccharide biomarker, is released in body fluids including urine. Urine is an abundant, safely collected specimen, well-suited for point-of-care (POC) testing, which could play an increasing role in screening for early disease. Our main objective was to demonstrate GM antigenuria as a clinically relevant biological phenomenon in IA and establish proof-of-concept that it could be translated to POC diagnosis. Utilizing a novel IgM monoclonal antibody (MAb476) that recognizes GM-like antigens from Aspergillus and other molds, we demonstrated antigenuria in an experimental animal IA model (guinea pig), as well as in human patients. In addition, we investigated the chemical nature of the urinary excreted antigen in human samples, characterized antigen detection in urine by immunoassays, described a putative assay inhibitor in urine, and indicated means of alleviation of the inhibition. We also designed and used a lateral flow immunochromatographic assay to detect urinary excreted antigen in a limited number of IA patient urine samples. In this study, we establish that POC diagnosis of IA based on urinary GM detection is feasible. Prospective studies will be necessary to establish the performance characteristics of an optimized device and define its optimal clinical use.
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影响因子：
4.3
作者：
labbe, Annie-Claude;Su, Shi Hann;Roy, Jean
通讯作者：
Roy, Jean
DOI：
10.1007/bf01971310
发表时间：
1994-07-01
影响因子：
4.5
作者：
ANSORG, R;VONHEINEGG, EH;RATH, PM
通讯作者：
RATH, PM
影响因子：
4.1
作者：
Ikuta, K;Shibata, N;Okawa, Y
通讯作者：
Okawa, Y
影响因子：
6.3
作者：
Asgeirsson, D.;Venturoli, D.;Rippe, C.
通讯作者：
Rippe, C.
DOI：
10.1086/652768
发表时间：
2010-06-15
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Baddley JW;Andes DR;Marr KA;Kontoyiannis DP;Alexander BD;Kauffman CA;Oster RA;Anaissie EJ;Walsh TJ;Schuster MG;Wingard JR;Patterson TF;Ito JI;Williams OD;Chiller T;Pappas PG
通讯作者：
Pappas PG