Regulation of FLC nuclear import by coordinated action of the NUP62-subcomplex and importin β SAD2
Regulation of FLC nuclear import by coordinated action of the NUP62-subcomplex and importin β SAD2
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DOI：
10.1111/jipb.13540
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发表时间：
2023
影响因子：
11.4
通讯作者：
Hu Honghong
中科院分区：
文献类型：
--
作者：
Chen Gang;Xu Danyun;Liu Qing;Yue Zhichuang;Dai Biao;Pan Shujuan;Chen Yongqiang;Feng Xinhua;Hu Honghong
Flowering locus C (FLC) is a central transcriptional repressor that controls flowering time. However, how FLC is imported into the nucleus is unknown. Here, we report that Arabidopsis nucleoporins 62 (NUP62), NUP58, and NUP54 composed NUP62-subcomplex modulates FLC nuclear import during floral transition in an importin α-independent manner, via direct interaction. NUP62 recruits FLC to the cytoplasmic filaments and imports it into the nucleus through the NUP62-subcomplex composed central channel. Importin β supersensitive to ABA and drought 2 (SAD2), a carrier protein, is critical for FLC nuclear import and flower transition, which facilitates FLC import into the nucleus mainly through the NUP62-subcomplex. Proteomics, RNAseq, and cell biological analyses indicate that the NUP62-subcomplex mainly mediates the nuclear import of cargos with unconventional nuclear localization sequences (NLSs), such as FLC. Our findings illustrate the mechanisms of the NUP62-subcomplex and SAD2 on FLC nuclear import process and floral transition, and provide insights into the role of NUP62-subcomplex and SAD2 in protein nucleocytoplasmic transport in plants.
