Chromosome 7-Centric Analysis of Proteomics Data from a Panel of Human Colon Carcinoma Cell Lines
Chromosome 7-Centric Analysis of Proteomics Data from a Panel of Human Colon Carcinoma Cell Lines
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DOI：
10.1021/pr300906y
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发表时间：
2013-01-01
影响因子：
4.4
通讯作者：
Nice, Edouard
中科院分区：
文献类型：
--
作者：
Fanayan, Susan;Smith, Joshua T.;Nice, Edouard
在这篇手稿中，我们描述了一个全面的蛋白质组学分析的样品，包括总裂解物，膜，分泌组，外泌体组分从一个面板的结直肠癌细胞系的鸟枪蛋白质组学方法。我们将以两种不同的格式对我们的蛋白质组学数据进行分析。首先，我们将讨论我们的数据的传统分析，其中我们使用各种蛋白质组学数据分析工具识别许多癌症相关蛋白。在第二种方法中，我们使用染色体格式来组织7号染色体上的蛋白质组数据，允许识别癌症相关基因簇，其边界由不同染色体上的物理接近度定义。
In this manuscript, we describe a shotgun proteomics approach for a comprehensive proteomic analysis of samples including total lysates, membrane, secretome, and exosome fractions from a panel of colorectal cancer cell lines. We will present an analysis of our proteomics data in two alternative formats. First we will discuss a traditional analysis of our data, in which we identify a number of cancer-associated proteins using various proteomic data analysis tools. In a second approach, we use a chromosome format to organize the proteomic data on chromosome 7, allowing the identification of clusters of cancer-associated genes with boundaries defined by physical proximity on different chromosomes.