Identification of an antigenic determinant of mouse lactate dehydrogenase C4.
Identification of an antigenic determinant of mouse lactate dehydrogenase C4.
复制标题
小鼠乳酸脱氢酶 C4 抗原决定簇的鉴定。
DOI：
10.1016/0161-5890(82)90269-3
复制
发表时间：
1982
影响因子：
3.6
通讯作者：
Goldberg,E
中科院分区：
文献类型：
--
作者：
Gonzales-Prevatt,V;Wheat,TE;Goldberg,E
一种带有精子特异性乳酸脱氢酶C4同工酶（LDH-C4）抗原决定簇的肽段已从整个蛋白质的胰蛋白酶消化物中分离出来。该肽包含残基152-159（MC 152 -159），与兔抗小鼠LDH-C4反应。用与牛血清白蛋白偶联的合成MC 152 - 159免疫家兔，诱导对该肽具有特异性的免疫应答。抗MC 152 - 159 IgG结合125 I标记的小鼠LDH-C4和竞争实验证明了这种抗原-抗体反应的特异性。
A peptide bearing an antigenic determinant of the sperm-specific lactate dehydrogenase C4isozyme (LDH-C4) has been isolated from a tryptic digest of the whole protein. This peptide, comprising residues 152–159 (MC152–159), reacts with rabbit anti-mouse LDH-C4. Immunization of rabbits with synthetic MC152–159conjugated to bovine serum albumin induces an immune response which is specific for the peptide. Anti-MC152–159IgG binds125I-labeled mouse LDH-C4and competition experiments demonstrate the specificity of this antigen-antibody reaction.