Prawn lipocalin: characterization of a color shift induced by gene knockdown and ligand binding assay.
Prawn lipocalin: characterization of a color shift induced by gene knockdown and ligand binding assay.
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DOI：
10.1002/jez.706
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
Fan Yang;Mo-Ran Wang;Yun Ma;W. Ma;Wei-Jun Yang
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文献类型：
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作者：
Fan Yang;Mo-Ran Wang;Yun Ma;W. Ma;Wei-Jun Yang
脂质运载蛋白家族在类固醇、类胡萝卜素、类维生素A和其他小疏水分子的转运中起作用。最近，脂质运载蛋白（MrLC）从罗氏沼虾中分离出来，其表达随蜕皮周期而变化。在这项研究中，通过RNA干扰（RNAi）敲低MrLC基因，导致整个甲壳的体色从蓝色转变为橘红色。通过免疫金电子显微镜，MrLC被发现与脂滴共定位在皮下脂肪组织中，发现在MrLC敲除对虾中显著减少，其中相对脂肪含量的减少也被量化。此外，在体外配体结合试验和体内天然配体检测中，发现MrLC特异性结合虾青素和蜕皮激素（20-羟基蜕皮激素）。这些结果表明，MrLC通过与虾青素的结合在甲壳动物的着色调节中发挥作用，并且可能还参与了甲壳动物蜕皮的调节。
The lipocalin family of proteins functions in the transport of steroids, carotenoids, retinoids, and other small hydrophobic molecules. Recently, a lipocalin (MrLC) was isolated from the prawn Macrobrachium rosenbergii and its expression varied with the molting cycle. In this study, knockdown of the MrLC gene by RNA interference (RNAi) was performed and resulted in a shift in body color from blue to orangish red over the entire carapace. By immune-gold electron microscopy, MrLC was found to co-localize with the lipid droplets in subepidermal adiose tissue that were found to be decreased dramatically in MrLC knockdown prawns, in which a reduction in relative fat content was also quantified. Furthermore, MrLC was found to specifically bind astaxanthin and molt hormone (20-hydroxyecdysone) in both in vitro ligand binding assay and in vivo native ligand detection. These results suggested that MrLC plays roles in the regulation of coloration through its association with astaxanthin and may also be involved in the regulation of molting in crustacean.