Response of stream fungi on decomposing leaves to experimental drying
Response of stream fungi on decomposing leaves to experimental drying
复制标题
溪流真菌对腐烂叶子的实验干燥反应
DOI：
10.1002/iroh.201902015
复制
发表时间：
2020
影响因子：
1.9
通讯作者：
Vessell, Brett P.
中科院分区：
文献类型：
--
作者：
Niyogi, Dev K.;Hu, Chia-Yi;Vessell, Brett P.
气候变化可能导致未来干旱的增加，这反过来又可能导致溪流干涸的时间增加。我们进行了一项实验，以测试干燥对真菌群落和微生物活性的影响腐烂的树叶从流。我们的实验设置包括将枫叶芯在小溪中浸泡2周以允许微生物的定殖。然后将叶片进行2周的四种处理：一种对照，其中叶片保持浸入流中，以及三种不同设置（田间、实验室和烘箱）中的干燥处理。然后将叶子放回无菌水中恢复2周。与对照相比，所有干燥处理后微生物呼吸都有所下降，其中烘干的叶子需要最长的时间才能恢复。浸泡恢复2周后，所有干燥处理彼此之间和对照之间的呼吸作用相似。通过聚合酶链反应扩增叶片真菌DNA，然后通过变性梯度凝胶电泳（DGGE）来评估叶片上的真菌群落。大多数治疗有非常相似的社区的基础上，从DGGE的植物类型，在干燥和恢复过程中变化不大浸泡控制相比。然而，烘干的叶子在恢复过程中有一个非常不同的群落。恢复后各处理间DGGE多态性的多样性和丰富度均无差异。总的来说，在我们的实验中，真菌群落似乎对短期干燥的影响有弹性，群落结构变化不大，复湿后活性恢复相对较快。
Climate change may lead to increased droughts in the future, which in turn may lead to increased periods of stream drying. We conducted an experiment to test the effects of drying on fungal communities and microbial activity on decaying leaves from a stream. Our experimental setup included immersion of maple leaf cores for 2 weeks in a small stream to allow for the colonization of microbes. Leaves were then subjected for 2 weeks to four treatments: one control, where leaves stayed immersed in the stream, and three drying treatments in different settings (field, lab, and oven). Leaves were then returned to sterile water for 2 weeks of recovery. Microbial respiration declined after all drying treatments compared to the control, with the oven‐dried leaves taking the longest time to recover. All drying treatments had similar respiration to each other and the control after 2 weeks of immersion recovery. Fungal communities on the leaves were assessed by polymerase chain reaction amplification of fungal DNA from leaves followed by denaturing gradient gel electrophoresis (DGGE). Most treatments had very similar communities based on phylotypes from DGGE, with little change during drying and recovery compared to immersion controls. However, the oven‐dried leaves had a very different community developing during recovery. There were no differences in diversity or richness of DGGE phylotypes among treatments after recovery. Overall, the fungal communities, in our experiment, appeared resilient to the effects of short‐term drying, with little change to community structure and relatively fast recovery in activity after rewetting.
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DOI：
--
发表时间：
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期刊：
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