Pressure-Loaded MSCs During Early Osteodifferentiation Promote Osteoclastogenesis by Increase of RANKL/OPG Ratio
Pressure-Loaded MSCs During Early Osteodifferentiation Promote Osteoclastogenesis by Increase of RANKL/OPG Ratio
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DOI：
10.1007/s10439-009-9638-9
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发表时间：
2009-04-01
影响因子：
3.8
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
Liu, Jun;Zhao, Zhihe;Wang, Jun
机械应力在骨重建中起重要作用。然而，目前还不清楚是否机械应力调节破骨细胞介导的间充质干细胞（MSCs）在初始骨分化。我们研究了静态和动态压力对骨髓间充质干细胞在早期骨分化过程中破骨诱导潜能的影响。TRAP染色观察破骨细胞生成情况。采用实时荧光定量RT-PCR分析核因子-κ B配体受体激活因子（RANKL）和骨保护素（OPG）基因的mRNA水平。结果表明，在初始成骨分化过程中暴露于任一压力的MSC促进破骨细胞的生成，并上调RANKL/OPG比率。MSC在不同的初始骨分化点对压力表现出不同的反应。在无压力暴露的情况下，原代MSCs诱导成骨后RANKL/OPG比值显著增加，这与以前的报道相矛盾。这些结果提示了骨重建对机械刺激的初始生物学反应的新机制。
Mechanical stress plays an important role in bone remodeling. However, it is still unclear whether mechanical stress regulates osteoclastogenesis mediated by mesenchymal stem cells (MSCs) during initial osteodifferentiation. We investigated the effects of static and dynamic pressures on osteoclast-inducing potential of MSCs during early osteodifferentiation. The osteoclastogenesis was examined using TRAP staining. The mRNA levels of receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) genes were analyzed using real-time RT-PCR. It was shown that MSCs exposed to either pressure during initial osteodifferentiation promoted osteoclastogenesis with the up-regulation of RANKL/OPG ratio. MSCs displayed diverse responses to pressures at different points of initial osteodifferentiation. The RANKL/OPG ratio was significantly increased after osteoinduction in the primary MSCs without pressures exposure, which contradicted the previous report. These results suggest novel mechanisms of the initial biological responses of bone remodeling upon mechanical stimuli.