Multi-component adsorption model for pellicle formation: The influence of salivary proteins and non-salivary phospho proteins on the binding of histatin 5 onto hydroxyapatite
Multi-component adsorption model for pellicle formation: The influence of salivary proteins and non-salivary phospho proteins on the binding of histatin 5 onto hydroxyapatite
复制标题
DOI：
10.1016/j.archoralbio.2005.06.003
复制
发表时间：
2006-02-01
影响因子：
3
通讯作者：
Oppenheim, FG
中科院分区：
文献类型：
--
作者：
Yin, A;Margolis, HC;Oppenheim, FG
获得性牙釉质膜是牙齿表面的一种保护膜，它是由唾液中的蛋白质选择性吸附而形成的。本研究的目的是研究人类获得性牙釉质薄膜的形成，使用体外羟基磷灰石（HA）模型和H-3-histatin 5，以便在多组分实验系统中准确测量histatin 5的结合。通过在与HA孵育之前将H-3-组蛋白抑制素5与一种未标记的蛋白质混合或通过首先将H-3-组蛋白抑制素5与已经用一组未标记的蛋白质（人白蛋白、唾液淀粉酶、溶菌酶、酸性PIF、statherin、statherin的N-末端片段和蛋黄胰蛋白酶）中的一种预包被的HA孵育来使用二元系统。通过将H-3-组胺素5与HA混合来使用三元系统，HA依次预包被有两种不同的未标记的蛋白质，包括重组组胺素1。结果表明，只有唾液中的他宁和蛋黄中的卵黄蛋白能显著促进histatin 5的吸附。还发现吸附的组胺素5的量作为用于预包被HA的抗组胺素和抑制素的量的函数增加，最高达到比在未处理的HA上观察到的高2 - 4倍的最大水平。这些数据表明，特定的蛋白质-蛋白质相互作用可能在体内膜形成中发挥重要作用。(c)2005爱思唯尔有限公司保留所有权利。
The acquired enamel pellicle formed by selective adsorption of proteins in whole saliva is a protective integument on the tooth surface. The purpose of the present study was to investigate the formation of human acquired enamel pellicle using an in vitro hydroxyapatite (HA) model and H-3-histatin 5 to allow accurate measurement of histatin 5 binding in a multi-component experimental system. A binary system was employed by mixing H-3-histatin 5 with one unlabeled protein prior to incubation with HA or by first incubating H-3-histatin 5 with the HA which had been pre-coated with one of a panel of unlabeled proteins (human albumin, salivary amytase, lysozyme, acidic PIFs, statherin, the N-terminal fragment of statherin, and egg yolk phosvitin). A ternary system was employed by mixing H-3-histatin 5 with HA sequentially pre-coated with two different unlabeled proteins, including recombinant histatin 1. The results showed that only salivary statherin and egg yolk phosvitin promote histatin 5 adsorption significantly. The amount of histatin 5 adsorbed was also found to increase as a function of the amount of phosvitin and statherin used to pre-coat HA up to a maximum level that was two- to four-fotd greater than that observed on untreated HA. These data suggest that specific protein-protein interactions may play important roles in pellicle formation in vivo. (c) 2005 Elsevier Ltd. All rights reserved.