Host-Induced Gene Silencing of the MAPKK Gene PsFUZ7 Confers Stable Resistance to Wheat Stripe Rust
Host-Induced Gene Silencing of the MAPKK Gene PsFUZ7 Confers Stable Resistance to Wheat Stripe Rust
复制标题
宿主诱导的 MAPKK 基因 PsFUZ7 基因沉默赋予小麦条锈病稳定的抗性
DOI：
10.1104/pp.17.01223
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发表时间：
2017-12-01
期刊：
影响因子：
7.4
通讯作者：
Guo, Jun
中科院分区：
文献类型：
--
作者：
Zhu, Xiaoguo;Qi, Tuo;Guo, Jun
RNA 干扰 (RNAi) 是一种强大的遗传工具，可加速植物生物技术研究并通过操纵靶基因表达来控制生物胁迫。然而，RNAi 在小麦中具有有效、持久地控制毁灭性条锈菌 Puccinia striiformis f 的潜力。 sp。迄今为止，小麦（Pst）的勘探工作仍处于很大程度上。为了解决这个问题，我们生成了表达 dsRNA 的转基因小麦 (Triticum aestivum) 系，该 dsRNA 靶向 Pst 的 PsFUZ7 转录本。我们分析了 PsFUZ7 和相关基因的表达以及转基因小麦品系的抗性性状。我们发现PsFUZ7是调节Pst感染和发展的重要致病因子。在两个独立的转基因小麦品系中稳定表达的 PsFUZ7 RNAi 构建体赋予对 Pst 的强抗性。 Pst菌丝发育受到强烈限制，并且在抗性反应中显着诱导植物细胞坏死。我们得出的结论是，RNA 分子从小麦植株运输到 Pst 可能会导致小麦和锈病病原体之间发生复杂的分子对话。此外，据我们所知，我们确认基于 RNAi 的作物保护方法可以用作针对小麦锈病病原体的新型控制策略。
RNA interference (RNAi) is a powerful genetic tool to accelerate research in plant biotechnology and control biotic stresses by manipulating target gene expression. However, the potential of RNAi in wheat to efficiently and durably control the devastating stripe rust fungus Puccinia striiformis f. sp. tritici (Pst) remained largely under explored so far. To address this issue, we generated transgenic wheat (Triticum aestivum) lines expressing dsRNA targeting PsFUZ7 transcripts of Pst. We analyzed expression of PsFUZ7 and related genes, and resistance traits of the transgenic wheat lines. We show that PsFUZ7 is an important pathogenicity factor that regulates infection and development of Pst. A PsFUZ7 RNAi construct stably expressed in two independent transgenic wheat lines confers strong resistance to Pst. Pst hyphal development is strongly restricted, and necrosis of plant cells in resistance responses was significantly induced. We conclude that trafficking of RNA molecules from wheat plants to Pst may lead to a complex molecular dialogue between wheat and the rust pathogen. Moreover, we confirm the RNAi-based crop protection approaches can be used, to our knowledge, as a novel control strategy against rust pathogens in wheat.