Species variation in organellar location and activity of L-pipecolic acid oxidation in mammals.
Species variation in organellar location and activity of L-pipecolic acid oxidation in mammals.
复制标题
哺乳动物细胞器位置和 L-哌啶酸氧化活性的物种差异。
DOI：
10.1007/bf00571266
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Rhead,WJ
中科院分区：
文献类型：
--
作者：
Mihalik,SJ;Rhead,WJ
用比以往更灵敏的检测系统研究了8种哺乳动物的l-吡喃甲酸氧化为α-氨基己二酸的反应。经Percoll梯度分离后，在兔、豚鼠、猪、狗和绵羊的组织中，活性集中在线粒体丰富的部分，其中豚鼠肾皮质的比活性最高。这些结果与在猕猴和人类中观察到的l-吡喃甲酸的过氧化体氧化形成对比(Mihalik和Rhead 1989；Mihalik等人。(1989年)。大鼠和小鼠都检测不到过氧化物体和线粒体-吡啶甲酸氧化水平。在大鼠中，无论是喂食氯贝特还是氯贝特和l-吡啶甲酸都不能诱导过氧化物酶的氧化活性。因此，在哺乳动物中，氧化吡喃甲酸的能力和这种氧化的细胞器位置都取决于物种。
The oxidation ofl-pipecolic acid to α-aminoadipic acid was studied in eight species of mammals using an assay system more sensitive than those previously employed. After percoll-gradient fractionation, activity was localized to the mitochondrial-enriched fractions in tissues from rabbit, guinea pig, pig, dog, and sheep, with guinea pig kidney cortex showing greatest specific activity. These results contrast with the peroxisomal oxidation ofl-pipecolic acid observed in macaques and man (Mihalik and Rhead 1989; Mihalik et al. 1989). Rats and mice had undetectable levels of both peroxisomal and mitochondriall-pipecolic acid oxidation. In the rat, peroxisomal oxidation activity was not induced by feeding with either clofibrate or clofibrate andl-pipecolic acid. Thus, among mammals, both the ability to oxidizel-pipecolic acid and the organellar location of this oxidation is species dependent.