An adhesion based approach for the detection of esophageal cancer.
An adhesion based approach for the detection of esophageal cancer.
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DOI：
10.1039/c8ib00132d
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发表时间：
2018-12-19
期刊：
影响因子：
--
通讯作者：
Goetz DJ
中科院分区：
文献类型：
--
作者：
Noori MS;Streator ES;Carlson GE;Drozek DS;Burdick MM;Goetz DJ
食管癌的5年生存率约为20%。这种令人沮丧的预后部分是由于食管癌通常出现在晚期。因此，迫切需要能够早期检测食管内的癌组织的测定。食管的管腔表面在转化位点表达特征分子，为开发检测食管内肿瘤生长的原位测定提供了途径。一个有吸引力的方法，越来越受到关注，是内窥镜给药的粒子与配体共轭的签名分子上存在的转化组织。在洗涤后检测食管内的颗粒将指示特征分子的存在，从而转化组织。在这项工作中，我们利用癌细胞和正常食管细胞，为这种利用配体偶联微球的方法提供体外原理证明，并证明需要，并提供工程化这项技术的框架。具体地，该研究（i）揭示了相对于正常细胞，在癌性食管细胞上特征分子的选择性增加的表达;（ii）证明了相对于正常细胞，配体缀合的微球与癌性食管细胞的选择性结合;（iii）证明了相对于正常食管细胞，癌性细胞的选择性识别高度依赖于测定的生物物理设计;和（iv）提倡利用来自细胞粘附领域的知识作为有效开发基于配体缀合颗粒的方案的指导，该方案寻求原位检测食管癌发生。内镜粘附试验中配体标记微球对食管癌细胞的选择性识别
Esophageal cancer has a 5-year survival rate of ~20%. This dismal prognosis is due, in part, to the fact that esophageal cancer often presents at a late stage. Thus, there is a critical need for assays that enable the early detection of cancerous tissue within the esophagus. The luminal surface of the esophagus expresses signature molecule(s) at sites of transformation providing an avenue for the development of in situ assays that detect neoplastic growth within the esophagus. An attractive approach, receiving increased attention, is the endoscopic administration of particles conjugated with ligands to signature molecules present on transforming tissue. Detection of the particles within the esophagus, post-washing, would indicate the presence of the signature molecule and thus transforming tissue. In this work, we utilized cancerous and normal esophageal cells to provide in vitro proof of principle for this approach utilizing ligand-conjugated microspheres and demonstrate the need, and provide the framework for, engineering this technology. Specifically, the study (i) reveals selective increased expression of signature molecules on cancerous esophageal cells relative to normal cells; (ii) demonstrates selective binding of ligand-conjugated microspheres to cancerous esophageal cells relative to normal cells; (iii) demonstrates that the selective recognition of cancerous, relative to normal esophageal cells, is highly dependent on the biophysical design of the assay; and (iv) advocates utilizing the knowledge from the field of cell adhesion as a guide for the effective development of ligand-conjugated particle-based schemes that seek to detect esophageal oncogenesis in situ. Selective recognition of esophageal cncer cells via ligand conjugated microspheres in an endoscopic adhesion assay
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影响因子：
29.4
作者：
Li M;Anastassiades CP;Joshi B;Komarck CM;Piraka C;Elmunzer BJ;Turgeon DK;Johnson TD;Appelman H;Beer DG;Wang TD
通讯作者：
Wang TD
影响因子：
29.4
作者：
Bird-Lieberman, Elizabeth L.;Dunn, Jason M.;Fitzgerald, Rebecca C.
通讯作者：
Fitzgerald, Rebecca C.
影响因子：
2.2
作者：
Kummitha, China Malakondaiah;Shirure, Venktesh S.;Goetz, Douglas J.
通讯作者：
Goetz, Douglas J.
影响因子：
64.8
作者：
ALON, R;HAMMER, DA;SPRINGER, TA
通讯作者：
SPRINGER, TA
影响因子：
3.5
作者：
Kirui, Dickson K.;Rey, Diego A.;Batt, Carl A.
通讯作者：
Batt, Carl A.