Whole genomes of deep-sea sponge-associated bacteria exhibit high novel natural product potential.
Whole genomes of deep-sea sponge-associated bacteria exhibit high novel natural product potential.
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全球抗菌素耐药性是一场可以改变现代医学面貌的健康危机。探索细菌衍生的新型抗微生物化合物的不同自然栖息地历来是一种成功的策略。深海为培养分类学上新颖的生物体和探索潜在的化学新空间提供了令人兴奋的机会。在这项研究中，草案基因组的12个细菌以前分离出的深海海绵现象carpenteri和Hertwigia的物种。专门的次级代谢产物的多样性进行了研究。此外，早期数据支持从许多这些菌株中产生抗菌抑制物质，包括对临床相关病原体鲍曼不动杆菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和金黄色葡萄球菌的活性。12个深海分离物的全基因组草案，其中包括四个潜在的新菌株：Psychrobacter sp. PP-21，Streptomyces sp. DK 15，Dietzia sp. PP-33和Micrococcus sp. M4 NT。在12个基因组草案中，检测到138个生物合成基因簇，其中一半以上与已知的BGC的相似性低于50%，这表明这些基因组为阐明新的次级代谢产物提供了令人兴奋的机会。探索属于放线菌门、假单胞菌门和芽孢杆菌门的细菌分离物，为那些从事抗生素发现工作的人提供了寻找新的化学多样性的机会。
Global antimicrobial resistance is a health crisis that can change the face of modern medicine. Exploring diverse natural habitats for bacterially-derived novel antimicrobial compounds has historically been a successful strategy. The deep-sea presents an exciting opportunity for the cultivation of taxonomically novel organisms and exploring potentially chemically novel spaces. In this study, the draft genomes of 12 bacteria previously isolated from the deep-sea sponges Phenomena carpenteri and Hertwigia sp. are investigated for the diversity of specialized secondary metabolites. In addition, early data support the production of antibacterial inhibitory substances produced from a number of these strains, including activity against clinically relevant pathogens Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Draft whole-genomes are presented of 12 deep-sea isolates, which include four potentially novel strains: Psychrobacter sp. PP-21, Streptomyces sp. DK15, Dietzia sp. PP-33, and Micrococcus sp. M4NT. Across the 12 draft genomes, 138 biosynthetic gene clusters were detected, of which over half displayed less than 50% similarity to known BGCs, suggesting that these genomes present an exciting opportunity to elucidate novel secondary metabolites. Exploring bacterial isolates belonging to the phylum Actinomycetota, Pseudomonadota, and Bacillota from understudied deep-sea sponges provided opportunities to search for new chemical diversity of interest to those working in antibiotic discovery.