An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
复制标题
分离柠檬源细胞外囊泡用于胃癌治疗的有效方法
DOI：
10.1186/s12951-020-00656-9
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发表时间：
2020-07-20
影响因子：
10.2
通讯作者：
Chen, Fuxiang
中科院分区：
文献类型：
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作者：
Yang, Meng;Liu, Xiaoyan;Chen, Fuxiang
背景植物源性细胞外囊泡（PDEV）具有巨大的临床应用潜力。超离心法被认为是制备PDEV的金标准方法，有效但耗时且高度依赖于仪器。因此，需要一种快速简便的方法来促进PDEVs的基础研究和临床应用。结果本研究将电泳技术与300 kDa截留透析袋（ELD）相结合，用于PDEVs的分离，节省时间，不需要特殊设备。利用ELD技术可以从柠檬汁中分离得到柠檬源性细胞外囊泡。纳米粒子跟踪分析和透射电子显微镜证实，该方法分离的完整囊泡具有类似的大小和数量的标准方法-超离心。LDEV可使胃癌细胞周期S期阻滞，并诱导细胞凋亡。LDEV对胃癌细胞的抗癌活性是通过产生活性氧来介导的。结论ELD是一种有效的分离LDEV的方法，无需特殊设备，可在常规生物学实验室进行。LDEV对胃癌具有一定的抗肿瘤活性，作为可食用的化疗药物载体具有很大的临床应用潜力。
BackgroundPlant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs.ResultsIn this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs.ConclusionsELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.