A highly specific pathogen-responsive promoter element from the immediate-early activated CMPG1 gene in Petroselinum crispum
A highly specific pathogen-responsive promoter element from the immediate-early activated CMPG1 gene in Petroselinum crispum
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DOI：
10.1046/j.1365-313x.2001.01015.x
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发表时间：
2001-04-01
期刊：
影响因子：
7.2
通讯作者：
Hahlbrock, K
中科院分区：
文献类型：
--
作者：
Kirsch, C;Logemann, E;Hahlbrock, K
在从病原体诱导子感知到防御相关基因激活的复杂信号网络中，一些立即早期响应基因可能在下游转录调控中发挥关键作用。我们已经鉴定出欧芹 (Petroselinum Criscum) EL117 基因是一种特别快速响应的基因，具有一种新型的包含 W 盒的激发子响应启动子元件 E17。转基因拟南芥植物中病原体感染位点的高度选择性 E17 介导的报告基因表达证明了该启动子元件在设计抗性育种新策略以及进一步分析防御相关基因激活机制的早期组成部分方面的潜力。 EL117基因编码的蛋白质表现出已建立的转录因子的各种结构特征，并且根据定义一类新出现的植物特异性蛋白质的前四个严格保守的氨基酸，被指定为CMPG蛋白质。
Within the complex signalling network from pathogen-derived elicitor perception to defense-related gene activation, some immediate-early responding genes may have pivotal roles in downstream transcriptional regulation. We have identified the parsley (Petroselinum crispum) EL117 gene as a particularly fast-responding gene possessing a new type of W box-containing, elicitor-responsive promoter element, E17. Highly selective E17-mediated reporter gene expression at pathogen infection sites in transgenic Arabidopsis thaliana plants demonstrated the potential of this promoter element for designing new strategies in resistance breeding as well as for further analysis of the early components of defense-related gene activation mechanisms. The protein encoded by the EL117 gene exhibits various structural characteristics of established transcription factors and is designated as a CMPG protein according to the first four strictly conserved amino acids defining a newly emerging class of plant-specific proteins.