Localisation of dehydroepiandrosterone sulphotransferase in adult rat brain
Localisation of dehydroepiandrosterone sulphotransferase in adult rat brain
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DOI：
10.1016/s0361-9230(98)00172-5
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发表时间：
1999-02-01
影响因子：
3.8
通讯作者：
Waring, RH
中科院分区：
文献类型：
--
作者：
Aldred, S;Waring, RH
在成年大鼠脑中评估了脱氢表雄酮磺基转移酶的存在和定位。研究人员对该酶的活性进行了研究，结果发现该酶的活性大约比大鼠肝脏中的酶活性低四倍。大鼠脑脱氢表雄酮磺基转移酶活性测定为 1.39 +/- 0.04 nm/mu g/min。使用蛋白质印迹法评估脱氢表雄酮磺基转移酶的蛋白表达。 35 kDa 处条带的存在表明成年大鼠大脑中存在这种酶。最后，通过免疫组织化学和评估大脑九个区域的活性来研究这种酶的定位。免疫组织化学显示海马、齿状回以及中脑大神经元呈阳性染色，表明存在脱氢表雄酮磺基转移酶。蛋白质印迹显示所有九个区域均存在条带，并且酶活性在海马、额叶皮层、丘脑和基底神经节中最高。这些结果意味着脱氢表雄酮和硫酸脱氢表雄酮 (DHEAS) 在脑功能中具有明确的作用，并且是独立于外周内分泌系统原位产生的。 (C) 1999 爱思唯尔科学公司。
The presence and localisation of dehydroepiandrosterone sulphotransferase was assessed in adult rat brain. The activity of the enzyme was investigated and was found to be approximately four times less than that in rat liver. Rat brain dehydroepiandrosterone sulphotransferase activity was measured as 1.39 +/- 0.04 nm/mu g/min. The protein expression of dehydroepiandrosterone sulphotransferase was evaluated using Western blotting. The presence of a band at 35 kDa signified the presence of this enzyme in adult rat brain. Finally, the localisation of this enzyme was investigated by immunohistochemistry and by assessing activity in nine regions of brain. Immunohistochemistry showed positive staining in the hippocampus and the dendate gyrus, and in the large neurons of the midbrain, indicating the presence of dehydroepiandrosterone sulphotransferase. Western blotting showed the presence of a band in all nine regions, and enzyme activity was highest in the hippocampus, frontal cortex, thalamus and basal ganglia. These results imply that dehydroepiandrosterone and dehydroepiandrosterone sulphate (DHEAS) have a defined role in brain function and are produced in situ independently of the peripheral endocrine system. (C) 1999 Elsevier Science Inc.