A previously unclassified trypanosomatid responsible for human cutaneous lesions in Martinique (French West Indies) is the most divergent member of the genus Leishmania ss
A previously unclassified trypanosomatid responsible for human cutaneous lesions in Martinique (French West Indies) is the most divergent member of the genus Leishmania ss
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DOI：
10.1017/s0031182001008927
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发表时间：
2002-01-01
期刊：
影响因子：
2.4
通讯作者：
Dedet, JP
中科院分区：
文献类型：
--
作者：
Noyes, H;Pratlong, F;Dedet, JP
马提尼克岛报告了两例类似于利什曼原虫寄生虫引起的皮肤病变。从这些病变分离的寄生虫不像利什曼原虫参考菌株同工酶分析和电子显微镜，并被假定为单菌锥虫，通常只感染无脊椎动物。这两种菌株现在已经通过同工酶分析重新分型，发现彼此相同，并且与所有其他利什曼原虫物种有远亲关系。获得了18S核糖体RNA基因的序列以及DNA聚合酶α和RNA聚合酶II最大亚基基因的部分序列。这些序列表明马提尼克寄生虫与L. Enriettii和基础的所有其他尤利什曼原虫。然而，支持的立场基础的所有尤利什曼原虫和集群与L。enriettii是低的。马提尼克寄生虫可能与L.（利什曼原虫）或利什曼原虫L.（Viannia）或在有或没有L. enriettii。
Two cases of skin lesions similar to those caused by Leishmania parasites have been reported from Martinique. Parasites isolated from these lesions were unlike Leishmania reference strains by isoenzyme analysis and electron microscopy and were assumed to be monoxenous trypanosomatids which normally only infect invertebrates. Both strains have now been retyped by isoenzyme analysis and found to be identical to each other and distantly related to all other Leishmania species. The sequence of the 18S ribosomal RNA gene and partial sequences of the DNA polymerase alpha and RNA polymerase I I largest subunit genes were obtained. These sequences indicated that the Martinique parasites clustered with L. enriettii and were basal to all other euleishmania. However, support for both the position basal to all euleishmania and the clustering with L. enriettii was low. The Martinique parasites may cluster with L. (Leishmania) or L. (Viannia) or form a novel clade within the euleishmania either with or without L. enriettii.