Protection against Vibrio alginolyticus in pearl gentian grouper (♀Epinephelus fuscoguttatus x ♂Epinephelus lanceolatu) immunized with an acfA-deletion live attenuated vaccine
Protection against Vibrio alginolyticus in pearl gentian grouper (♀Epinephelus fuscoguttatus x ♂Epinephelus lanceolatu) immunized with an acfA-deletion live attenuated vaccine
复制标题
使用 acfA 缺失减毒活疫苗免疫的珍珠龙胆石斑鱼（雌性褐斑石斑鱼 x 雄性披针石斑鱼）对溶藻弧菌的保护
DOI：
10.1016/j.fsi.2018.12.030
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发表时间：
2019-03-01
影响因子：
4.7
通讯作者：
Jian, Jichang
中科院分区：
文献类型：
--
作者：
Chen, Yanyan;Cai, Shuanghu;Jian, Jichang
溶藻弧菌（Vibrio alginolyticus）是一种常见的机会致病菌，严重危害全球水产养殖业的发展和人类健康。在这项研究中，一个Δ acfA突变株和互补的Δ acfA突变体（C-acfA）的构建。以珍珠龙胆石斑鱼（Epinealumus fuscoguttatus × Epinealumus lanceolatu）为试验动物，研究了Δ acfA突变体的毒力变化，并评价了其作为减毒活疫苗的潜力。结果表明，Δ acfA突变体引起了高抗体滴度和显着降低的能力，在肠道的珍珠龙胆石斑鱼的殖民。接种Delta acfA突变体的石斑鱼对溶藻弧菌HY 9901强毒的耐受性更强，且未引起临床症状和明显的病理变化。用溶藻弧菌HY 9901攻毒后，Delta acfA突变体腹腔注射免疫的珍珠龙胆石斑鱼的相对存活率达到81.1%。用Delta acfA免疫的特异性抗体滴度显著高于PBS组。Delta acfA组抗体滴度呈1 ~ 4周上升，5 ~ 8周下降的趋势，第4周达到高峰。同时，免疫后IL-1 β、TNF-α、IL-16、IgM、CD 8 α和MHC-I α等免疫相关基因的表达水平均上调，表明Delta acfA能诱导珍珠龙胆石斑鱼产生有效持久的免疫应答，可能是一种预防溶藻弧菌感染的有效减毒活疫苗候选物。
Vibrio alginolyticus is well-known as an opportunistic Grain-negative pathogen, which endangers the development of global aquaculture as well as human health. In this study, a Delta acfA mutant strain and complementation of the Delta acfA mutant (C-acfA) were constructed. The Delta acfA mutant was tested in pearl gentian grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) to observe the changes in virulence and evaluate its potential as an attenuated live vaccine. The results showed that the Delta acfA mutant caused a high antibody titer and a significant reduction in the ability to colonize the intestine of pearl gentian grouper. Grouper vaccinated with Delta acfA mutant were more tolerant of the infection by virulent V. alginolyticus HY9901 without inducing clinical symptoms and obvious pathological changes. The relative percent survival value of pearl gentian grouper vaccinated with Delta acfA mutant intraperitoneal injection reached 81.1% after challenging with V. alginolyticus HY9901. The specific antibody titers immunized with Delta acfA was significantly higher than that in the PBS group. The antibody titer of Delta acfA group displayed the tendency of rising up from the first to fourth week and declining from fifth to eighth week and reached the peak at the fourth week. In the meanwhile, the expression level of genes associated with immunity, including IL-1 beta, TNF-alpha, IL-16, IgM, CD8 alpha and MHC-I alpha, was up-regulated after vaccination, indicating that the Delta acfA can induce effective and durable immune response in pearl gentian grouper and it may be an effective attenuated live vaccine candidate for the prevention of infections by V. alginolyticus.