UNSTIMULATED RELEASE OF ENDOTHELIUM DERIVED RELAXING FACTOR IS INDEPENDENT OF MITOCHONDRIAL ATP GENERATION
UNSTIMULATED RELEASE OF ENDOTHELIUM DERIVED RELAXING FACTOR IS INDEPENDENT OF MITOCHONDRIAL ATP GENERATION
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DOI：
10.1093/cvr/21.8.565
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发表时间：
1987-08-01
影响因子：
10.8
通讯作者：
HENDERSON, AH
中科院分区：
文献类型：
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作者：
GRIFFITH, TM;EDWARDS, DH;HENDERSON, AH
采用兔主动脉条研究线粒体抑制剂对基础（未刺激）内皮依赖性舒张的影响。由于血红蛋白抑制和环GMP磷酸二酯酶抑制剂，MB 22948，放大内皮依赖性舒张，它们被用来提供内皮衍生舒张因子（EDRF）的基础活性的证据。基础活动没有抑制孵育与任何三个不同的线粒体ATP生成抑制剂。因此，基础EDRF产生的机制可能不同于刺激EDRF产生的机制，后者被这些线粒体抑制剂消除。
Rabbit aortic strips were used to investigate the effect of mitochondrial inhibitor on basal (unstimulated) endothelium dependent relaxation. Since haemoglobin inhibits and the cyclic GMP phosphodiesterae inhibitor, MB22948, amplifies endothelium dependent relaxation they were used to provide evidence of basal activity of endothelium derived relaxing factor (EDRF). Basal activity was not inhibited by incubation with any of three differently acting inhibitors of mitochondrial ATP generation. The mechanism underlying basal EDRF production may thus differ from that of stimulated EDRF production, which is abolished by these mitochondrial inhibitors.