A digital image-based flow-batch analyzer for determining Al(III) and Cr(VI) in water
A digital image-based flow-batch analyzer for determining Al(III) and Cr(VI) in water
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DOI：
10.1016/j.microc.2012.03.029
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发表时间：
2013-07-01
影响因子：
4.8
通讯作者：
Silva, Edvan C.
中科院分区：
文献类型：
--
作者：
Andrade, Stefani Jury E.;Lima, Marcelo B.;Silva, Edvan C.
本研究提出了一种基于数字图像的流动批次分析仪(DIB-FBA)，用于天然水中铝(III)和铬(VI)的测定。使用了带有CCD传感器和RGB数据的网络摄像头。铝的测定方法是基于铝(III)离子、槲皮素和十六烷基三甲基溴化铵(CTAB)的反应，在醋酸盐缓冲介质(pH 5.5)中生成黄色的络合物。铬的测定是基于铬(VI)离子与1，5-二苯基碳酰二肼(DPC)的反应，产物为紫罗兰色的络合物。数字图像显示了在每次分析中形成的络合物所吸收的辐射的颜色。用RGB数据建立解析曲线。所有标准溶液都是在线配制的，所有分析过程只需在DIB-FBA控制软件中改变操作参数即可完成。工作范围：AI(Ⅲ)10~600mg L~(-1)，r=0.9994(n=5)；Cr(VI)10~300mg L~(-1)，r=0.999(n=5)；Al(III)和Cr(VI)的相对标准偏差(RSD%)分别为1.5%和137 h(-1)，进样率分别为1.7%和134 h(-1)。与参考方法相比，在95%的置信度水平下应用配对t检验，没有观察到统计学上的显著差异。(C)2012爱思唯尔B.V.保留所有权利。
This study proposes a digital image-based flow-batch analyzer (DIB-FBA) for aluminum (III) and chromium (VI) determinations in natural water. A webcam with a CCD sensor and RGB data are used. The method for determining aluminum is based on an Al(III) ion, quercetin, and cetyltrimethylammonium bromide (CTAB) reaction which yields a yellow colored complex in an acetate buffer medium (pH 5.5). The determination of chromium is based on a Cr(VI) ion with 1,5-diphenylcarbazide (DPC) reaction whose product is a violet colored complex. Digital images present the color of the emergent (complementary) radiation to that absorbed by the complexes formed in each analysis. RGB data were employed to build the analytical curves. All standard solutions were prepared in-line, and all analytical processes were completed by simply changing the operational parameters in the DIB-FBA control software. The working ranges were from 10 to 600 mu g L-1 for AI(III) with r=0.9994 (n=5), and 10 to 300 mu g L-1 for Cr(VI) with r=0.999 (n=5), the limits of detection were 3.97 and 2.65 mu g L-1 respectively for AI(III) and Cr(VI). The relative standard deviation (RSD %) and sampling rate were estimated at 1.5% and 137 h(-1) and at 1.7% and 134 h(-1) for Al (III) and Cr(VI), respectively. Comparing with the reference method, no statistically significant differences were observed when applying the paired t-test at a 95% confidence level. (C) 2012 Elsevier B.V. All rights reserved.