Involvement of neuronal, inducible and endothelial nitric oxide synthases in capsaicin-induced muscle hypersensitivity.
Involvement of neuronal, inducible and endothelial nitric oxide synthases in capsaicin-induced muscle hypersensitivity.
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DOI：
10.1016/j.ejpain.2008.11.009
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Ro JY
中科院分区：
文献类型：
--
作者：
Lee JS;Zhang Y;Ro JY
一氧化氮与脊髓系统痛觉过敏的发展有关，但尚未在三叉神经系统中进行系统研究，特别是在炎症性肌肉疼痛的情况下。在这项研究中，我们研究了中央释放一氧化氮在口面部肌肉疼痛发病机制中的功能作用。具体来说，我们分别检测了神经元型、诱导型和内皮型一氧化氮合酶nNOS、iNOS和eNOS在急性炎症条件下介导咬肌超敏反应中的作用。研究了雄性大鼠咬肌注射辣椒素后尾侧亚核（Vc）中nNOS、iNOS和eNOS蛋白表达的时间依赖性变化。辣椒素刺激后30-60 min，三种一氧化氮合酶的表达均显著上调，与辣椒素诱导咬肌超敏反应的发生时间一致。各NOS抑制剂预处理均可显著减轻咬肌超敏反应。这些数据表明，Vc中的所有三个NOS在颅面肌痛觉过敏的发展中都具有重要的功能，并表明这三个NOS在正常和病理条件下密切协调，调节一氧化氮水平。
Nitric oxide, which has been implicated in the development of hyperalgesia in the spinal system, has not been systematically studied in the trigeminal system, especially in the context of inflammatory muscle pain condition. In this study, we investigated the functional role of centrally released nitric oxide in the pathogenesis of orofacial muscle pain. Specifically, we examined the contribution of neuronal, inducible and endothelial nitric oxide synthases, nNOS, iNOS and eNOS, respectively, in mediating masseter hypersensitivity under acute inflammatory condition. Time-dependent changes in nNOS, iNOS and eNOS protein expression in the subnucleus caudalis (Vc) were assessed following capsaicin injection in the masseter muscle of male Sprague Dawley rats. The expression of all three nitric oxide synthases was significantly up-regulated 30–60 min following capsaicin stimulation, which paralleled the time course of the development of capsaicin-induced masseter hypersensitivity. Pretreatment with each NOS inhibitor significantly attenuated the masseter hypersensitivity. These data showed that all three NOS in the Vc are functionally important for the development of craniofacial muscle hyperalgesia and suggest that the three NOS are closely orchestrated to regulate the level of nitric oxide under normal and pathologic conditions.
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