TUMOR-LOCALIZATION OF RADIOLABELED ANTIBODIES AGAINST CARCINOEMBRYONIC ANTIGEN IN PATIENTS WITH CARCINOMA - A CRITICAL-EVALUATION
TUMOR-LOCALIZATION OF RADIOLABELED ANTIBODIES AGAINST CARCINOEMBRYONIC ANTIGEN IN PATIENTS WITH CARCINOMA - A CRITICAL-EVALUATION
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DOI：
10.1056/nejm198007033030102
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发表时间：
1980-01-01
影响因子：
158.5
通讯作者：
ALBERTO, P
中科院分区：
文献类型：
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作者：
MACH, JP;CARREL, S;ALBERTO, P
将纯化的、[131I]标记的抗癌胚抗原羊抗体注射到27例人癌裸鼠体内。患者在不同的时间间隔用闪烁相机进行扫描。11例患者于注射后48h在肿瘤内检测到放射性。计算机化的血池放射性减影在阳性病例中提供了更清晰的图像，但在16例患者中，扫描结果仍然可疑或阴性。为了研究[131I]抗体定位的特异性，部分患者同时注射了[125I]标记的正常Ig[免疫球蛋白]G，用闪烁计数法测定了肿瘤和手术后恢复的正常组织中的这两种同位素。仅抗CEA抗体定位于肿瘤。然而，肿瘤中抗体衍生的总放射性仅为注射剂量的0.001左右。尽管目前证明了这种特异性，但这种肿瘤检测方法还没有在临床上使用。
Purified, [131I]-labeled goat antibodies against carcinoembryonic antigen, which localize in human carcinoma in nude mice, were injected into 27 patients with carcinoma. Patients were scanned with a scintillation camera at various intervals. In 11 patients, radioactivity was detectable in the tumor 48 h after injection. Computerized subtraction of blood-pool radioactivity provided clearer pictures in positive cases, but in 16 patients the scans remained doubtful or negative. To study the specificity of [131I]-antibody localization, some patients were given simultaneous injections of [125I]-labeled normal Ig[immunoglobulin]G. Both isotopes were measured by means of scintillation counting in tumors and normal tissues recovered after surgery. Only the anti-CEA antibodies localized in tumors. However, the total antibody-derived radioactivity in the tumor was only about 0.001 of the injected dose. Despite the present demonstration of specificity, this method of tumor detection is not yet clinically useful.