ULTRASTRUCTURE OF THE ENCAPSULATION OF PLASMODIUM-CYNOMOLGI (B-STRAIN) ON THE MIDGUT OF A REFRACTORY STRAIN OF ANOPHELES-GAMBIAE
ULTRASTRUCTURE OF THE ENCAPSULATION OF PLASMODIUM-CYNOMOLGI (B-STRAIN) ON THE MIDGUT OF A REFRACTORY STRAIN OF ANOPHELES-GAMBIAE
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DOI：
10.2307/3282053
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发表时间：
1988-06-01
影响因子：
1.3
通讯作者：
COLLINS, FH
中科院分区：
文献类型：
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作者：
PASKEWITZ, SM;BROWN, MR;COLLINS, FH
使用透射电子显微镜，我们研究了猿猴疟疾寄生虫食蟹猴疟原虫在难治性蚊子冈比亚按蚊菌株中的封装情况。当动动子穿透蚊子中肠上皮并停留在基底膜和基底层之间后，一种电子致密的黑色素样物质开始在寄生虫周围凝聚。早在血粉后 16 小时就发现了完全包裹的寄生虫。黑色素样物质的颗粒常常从基底膜迷路细胞外空间的液体中凝结到寄生虫上。黑色素颗粒似乎也从血淋巴凝结到寄生虫下方的基底层上。此外，在位于寄生虫旁边或包围寄生虫的中肠细胞中经常发现成组的小管、囊泡和膜轮。这些结构的电子密度异常高，可能与黑化有关。在完整的胶囊附近很少观察到血细胞，并且血细胞或其残余物都不是胶囊的组成部分。在包囊的后期，寄生虫出现异常，并且经常被黑色素浸润。虽然晚期胶囊通常位于基底，但偶尔在中肠顶端边界附近观察到被膜包围的完整胶囊。吸血后 1 至 6 天，在中肠腔中发现了与细胞碎片相关的其他胶囊。
Using transmission electron microscopy, we investigated the encapsulation of the simian malaria parasite, Plasmodium cynomolgi, in a refractory strain of the mosquito, Anopheles gambiae. After the ookinete penetrates the mosquito midgut epithelium and lodges between the basal membrane and the basal lamina, an electron-dense, melanin-like substance begins to coalesce around the parasite. Completely encapsulated parasites were found as early as 16 hr after the blood meal. Granules of the melanin-like substance often appeared to condense onto the parasite from the fluid in the extracellular spaces of the basal membrane labyrinth. Melanin granules also appeared to condense from the hemolymph onto the basal lamina underlying the parasite. In addition, groups of tubules, vesicles, and membranous whorls often were found in midgut cells that were located next to or were enclosing parasites. These structures were unusually electron-dense, and may have been associated with melanization. Hemocytes rarely were observed near completed capsules and neither hemocytes nor their remnants were components of the capsules. During later stages of encapsulation, parasites appeared abnormal and often were infiltrated with melanin. Although late-stage capsules were usually located basally, completed capsules enclosed by membranes were occasionally observed near the apical border of the midgut. Other capsules, associated with cellular debris, were found in the lumen of the midgut from 1 to 6 days after the blood meal.