Development of EST-PCR markers and monitoring their intrapopulational genetic variation in Picea abies (L.) Karst.
Development of EST-PCR markers and monitoring their intrapopulational genetic variation in Picea abies (L.) Karst.
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DOI：
10.1007/s001220000501
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发表时间：
2001-12
影响因子：
5.4
通讯作者：
R. Schubert;G. Mueller-Starck;R. Riegel
中科院分区：
文献类型：
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作者：
R. Schubert;G. Mueller-Starck;R. Riegel
本文报道了欧洲针叶林树种挪威云杉[Piceabies（L.）喀斯特），包括公共电子数据库中相似性搜索的结果。序列随后用于设计特异性引物对和基于PCR的基因组片段扩增。7对引物在18棵树中扩增出多态性EST-PCR标记。通过分析单株后代和研究单倍体胚乳与二倍体组织之间的分离，验证了它们的遗传模式。其中6个标记为共显性遗传，1个标记为显性遗传。6个共显性EST标记的变化进行了测试，基因型110个随机选择的树木在巴伐利亚挪威云杉种群。为了比较，在18个酶编码基因位点对相同的树进行基因分型。EST标记的等位基因数为3.33个，同工酶基因标记的等位基因数为3.00个。在一般情况下，更均匀的频率分布和更大的种群内变异，包括观察到的杂合性的趋势，表明更多的EST标记比同工酶基因标记。概述了这些新开发的EST-PCR标记在群体遗传学和生态遗传学方面的优势。
Fifteen cDNA sequences are reported for the European coniferous forest tree species Norway spruce [Picea abies(L.) Karst.], including the results of similarity searches in public electronic databases. The sequences were subsequently employed for the design of specific primer pairs and PCR-based amplification of genomic fragments. For seven primer pairs, polymorphic EST-PCR markers were identified among 18 trees. Their mode of inheritance was verified by analysing single-tree offspring and studying segregation among haploid endosperms in comparison to diploid tissue. Codominant inheritance was indicated for six markers, while one marker was apparently dominant. Variation of the six codominant EST markers was tested by genotyping 110 randomly selected trees in a Bavarian Norway spruce population. For comparison, the same trees were genotyped at 18 enzyme coding gene loci. There were 3.33 alleles per locus for EST markers and 3.00 for isoenzyme gene markers. In general, a trend to more even frequency distributions and larger intrapopulational variation, including observed heterozygosities, was indicated more for EST markers than for isoenzyme gene markers. The benefits of these newly developed EST-PCR markers are outlined with respect to population genetics and ecological genetics.