A fiber-optic system for dual-modality photoacoustic microscopy and confocal fluorescence microscopy using miniature components.
A fiber-optic system for dual-modality photoacoustic microscopy and confocal fluorescence microscopy using miniature components.
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DOI：
10.1016/j.pacs.2013.07.001
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发表时间：
2013-05-01
期刊：
影响因子：
7.9
通讯作者：
Wang, Xueding
中科院分区：
文献类型：
--
作者：
Chen, Sung-Liang;Xie, Zhixing;Guo, L Jay;Wang, Xueding
同时对细胞和微血管进行成像，有利于肿瘤血管生成和微环境的研究。设计并构建了基于光纤光声显微镜（PAM）和共聚焦荧光显微镜（CFM）的双模成像系统。为了探索这种全光器件在未来内窥镜应用的可行性，采用微机电系统（MEMS）扫描仪、微型物镜和小尺寸光学微环谐振器作为声探测器，试图满足小型化的要求。PAM和CFM的横向分辨率均为8.8 μm。实验测得PAM和CFM的轴向分辨率分别为19 μm和53 μm。离体动物膀胱组织实验表明，该系统在微血管和细胞结构成像方面均有良好的表现，提示该新型成像技术在改善膀胱癌的诊断和指导治疗方面具有潜力。
Imaging of the cells and microvasculature simultaneously is beneficial to the study of tumor angiogenesis and microenvironments. We designed and built a fiber-optic based photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) dual-modality imaging system. To explore the feasibility of this all-optical device for future endoscopic applications, a microelectromechanical systems (MEMS) scanner, a miniature objective lens, and a small size optical microring resonator as an acoustic detector were employed trying to meet the requirements of miniaturization. Both the lateral resolutions of PAM and CFM were quantified to be 8.8 μm. Axial resolutions of PAM and CFM were experimentally measured to be 19 μm and 53 μm, respectively. The experiments on ex vivo animal bladder tissues demonstrate the good performance of this system in imaging not only microvasculature but also cellular structure, suggesting that this novel imaging technique holds potential for improved diagnosis and guided treatment of bladder cancer.