Shaped DNA origami carrier nanopore translocation influenced by aptamer based surface modification.
Shaped DNA origami carrier nanopore translocation influenced by aptamer based surface modification.
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DOI：
10.1016/j.bios.2021.113658
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发表时间：
2021-09
影响因子：
12.6
通讯作者：
Taoli Ding;Jing Yang;Juan Wang;Victor Pan;Zuhong Lu;Yonggang Ke;Cheng Zhang
中科院分区：
文献类型：
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作者：
Taoli Ding;Jing Yang;Juan Wang;Victor Pan;Zuhong Lu;Yonggang Ke;Cheng Zhang
DNA折纸被广泛用作易位载体来辅助固态纳米孔分析，例如软线性折纸载体和异形折纸结构。在基于线性折纸载体的纳米孔传感中，分子修饰引起微小的结构和电荷变化，可以导致易位信号的显着变化，从而促进单分子传感。然而，对于固态（SS）纳米孔易位过程中表面修饰对异形DNA折纸结构的影响的理解仍然难以捉摸。在此，我们报道了一种使用适体/靶标结合的表面修饰策略，以影响成形折纸带载体通过SS纳米孔的易位。我们的测量表明，易位信号变化可以响应载体表面上的 ATP/适体结合，甚至响应空间分布和酶催化诱导的表面修饰。同时，结果还表明利用SS-纳米孔识别DNA折纸上微小的空间和电子变化的可能性。我们预计，表面适体结合影响的折纸易位策略可以在折纸载体辅助的 SS 纳米孔传感和检测中找到更多应用。
DNA origami is widely used as a translocation carrier to assist solid-state nanopore analysis, e.g., soft linear origami carrier and special-shaped origami structures. In the linear origami carriers based nanopore sensing, molecular modifications induced tiny structural and charge changes, can result in significant variations on translocation signals to facilitating single-molecule sensing. However, an understanding on the influences of surface modifications on special-shaped DNA origami structures during solid-state (SS) nanopores translocation is still far elusive. Herein, we reported a surface modification strategy using aptamer/target-binding to influence the translocation of the shaped origami ribbon carrier through SS-nanopore. Our measurements indicate that the translocation signal variations can respond to ATP/aptamer binding on the carrier surface, even to the surface modifications induced by spatial distributions and enzyme catalysis. Meanwhile, the results also suggest a possibility to identify small spatial and electronic changes on DNA origami by using SS-nanopore. We envision that the surface aptamer-binding influenced origami translocation strategy could find more applications in origami carrier assisted SS-nanopore sensing and detection.