Enzymatic site-selectivity enabled by structure-guided directed evolution
Enzymatic site-selectivity enabled by structure-guided directed evolution
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DOI：
10.1039/c7cc00368d
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发表时间：
2017-04-11
影响因子：
4.9
通讯作者：
Reetz, Manfred T.
中科院分区：
文献类型：
--
作者：
Wang, Jian-bo;Li, Guangyue;Reetz, Manfred T.
生物催化位点选择性（区域选择性）有机转化已经实践了几十年，但酶的传统局限性，狭窄的底物接受和经常观察到的选择性不足的程度一直持续到最近。随着定向进化的出现，有可能设计位点选择性以满足有机化学家的需求。本文综述了这一令人兴奋的研究领域的最新进展，选择的例子涉及P450单加氧酶，卤化酶和Baeyer-Villiger单加氧酶被精选用于说明目的。强调了酶和人造催化剂的互补性。
Biocatalytic site-selective (regioselective) organic transformations have been practiced for decades, but the traditional limitations of enzymes regarding narrow substrate acceptance and the often observed insufficient degree of selectivity have persisted until recently. With the advent of directed evolution, it is possible to engineer site-selectivity to suit the needs of organic chemists. This review features recent progress in this exciting research area, selected examples involving P450 monooxygenases, halogenases and Baeyer-Villiger monooxygenases being featured for illustrative purposes. The complementary nature of enzymes and man-made catalysts is emphasized.