Computational study of the activated O(H) state in the catalytic mechanism of cytochrome c oxidase.
Computational study of the activated O(H) state in the catalytic mechanism of cytochrome c oxidase.
复制标题
细胞色素c氧化酶催化机制中活化O(H)态的计算研究。
DOI：
10.1073/pnas.1220379110
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发表时间：
2013
影响因子：
11.1
通讯作者：
Wikström,Mårten
中科院分区：
文献类型：
--
作者：
Sharma,Vivek;Karlin,KennethD;Wikström,Mårten
Complex IV in the respiratory chain of mitochondria and bacteria catalyzes reduction of molecular oxygen to water, and conserves much of the liberated free energy as an electrochemical proton gradient, which is used for the synthesis of ATP. Photochemical electron injection experiments have shown that reduction of the ferric/cupric state of the enzyme’s binuclear hemea3/CuBcenter is coupled to proton pumping across the membrane, but only if oxidation of the reduced enzyme by O2immediately precedes electron injection. In contrast, reduction of the binuclear center in the “as-isolated” ferric/cupric enzyme is sluggish and without linkage to proton translocation. During turnover, the binuclear center apparently shuttles via a metastable but activated ferric/cupric state (OH), which may decay into a more stable catalytically incompetent form (O) in the absence of electron donors. The structural basis for the difference between these two states has remained elusive, and is addressed here using computational methodology. The results support the notion that CuB[II] is either three-coordinated in the OHstate or shares an OH−ligand with hemea3in a strained μ-hydroxo structure. Relaxation to state O is initiated by hydration of the binuclear site. The redox potential of CuBis expected, and found by density functional theory calculations, to be substantially higher in the OHstate than in state O. Our calculations also suggest that the neutral radical form of the cross-linked tyrosine in the binuclear site may be more significant in the catalytic cycle than suspected so far.
