BDNF and NT-3 induce intracellular Ca2+ elevation in hippocampal neurones.
BDNF and NT-3 induce intracellular Ca2+ elevation in hippocampal neurones.
复制标题
BDNF 和 NT-3 诱导海马神经元细胞内 Ca2+ 升高。
作者：
B. Berninger;D. E. Garcia;N. Inagaki;C. Hahnel;D. Lindholm
使用fura-2荧光显微镜在体外研究了神经营养素对大鼠海马神经元细胞内Ca2+水平的影响。 BDNF 和 NT-3（而非 NGF）使这些神经元中的细胞质 Ca2+ 浓度迅速增加十倍，达到 1 µM。此外，在一些神经元中，BDNF 和 NT-3 均引发 Ca2+ 反应，表明同一细胞中存在这些神经营养素的功能性受体。在这些培养物中，大约 80% 的海马神经元被全长 TrkB 抗体染色。 RNA 分析也证实了功能性 TrkB 的表达。这些结果证明海马神经元中存在 BDNF 和 NT-3 功能性受体。
The effects of neurotrophins on intracellular Ca2+ levels in rat hippocampal neurones were studied in vitro using fura-2 fluorescence microscopy. BDNF and NT-3, but not NGF, rapidly increased cytoplasmic Ca2+ concentrations in these neurones ten-fold to reach 1 microM. Moreover in some of the neurones both BDNF and NT-3 elicited Ca2+ responses, indicative of the presence of functional receptors for these neurotrophins in the same cell. In these cultures approximately 80% of the hippocampal neurones were stained with antibodies against full-length TrkB. The expression of functional TrkB was also confirmed by RNA analysis. These results demonstrate the presence of functional receptors for BDNF and NT-3 in hippocampal neurones.