An octakis-intercalating molecule
An octakis-intercalating molecule
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DOI：
10.1016/s0968-0896(00)00335-7
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发表时间：
2001-05-01
影响因子：
3.5
通讯作者：
Iverson, BL
中科院分区：
文献类型：
--
作者：
Murr, MM;Harting, MT;Iverson, BL
在这里，我们报告的合成和表征的polyintercalator与八个潜在的嵌入1，4，5，8-萘四甲酸二酰亚胺（NDI）的单位连接在一个头到尾的安排，通过肽接头。紫外光谱和粘度测量表明该分子与双链DNA结合，所有8个NDI单元同时插入。竞争透析和DNAse 1足迹研究揭示了对富含GC的DNA区域的偏好，圆二色性研究揭示了结合后B型DNA的显著变形。据我们所知，我们所谓的“八聚体”代表了第一种作为八聚体嵌入剂结合的嵌入剂，能够跨越至少16个碱基对的DNA。(C)2001爱思唯尔科技有限公司版权所有。
Herein we report the synthesis and characterization of a polyintercalator with eight potential intercalating 1,4,5,8-naphthalenetetracarboxylic diimide (NDI) units linked in a head-to-tail arrangement via a peptide linker. W spectroscopy and viscometry measurements indicated the molecule binds to double-stranded DNA with all eight NDI units intercalated simultaneously. Competition dialysis and DNAse 1 footprinting studies revealed a preference for GC-rich regions of DNA, and circular dichroism studies revealed significant distortion of B-form DNA upon binding. Our so-called 'octamer' represents, to the best of our knowledge, the first intercalator that binds as an octakis-intercalator, capable of spanning at least 16 base pairs of DNA. (C) 2001 Elsevier Science Ltd. All rights reserved.