CsMYB60 directly and indirectly activates structural genes to promote the biosynthesis of flavonols and proanthocyanidins in cucumber
CsMYB60 directly and indirectly activates structural genes to promote the biosynthesis of flavonols and proanthocyanidins in cucumber
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CsMYB60直接和间接激活结构基因促进黄瓜黄酮醇和原花青素的生物合成
DOI：
10.1038/s41438-020-0327-z
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发表时间：
2020-07-01
影响因子：
8.7
通讯作者：
Ren, Zhonghai
中科院分区：
文献类型：
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作者：
Li, Jialin;Luan, Qianqian;Ren, Zhonghai
黄酮醇和原花青素是黄瓜果实黑刺中的主要色素，CsMYB60是黄酮醇和原花青素生物合成的关键调控因子。然而，在黄瓜中，黄酮醇和PAs的组织分布模式以及CsMYB60调控的生物合成机制尚不清楚。在本研究中，我们明确了类黄酮在黄瓜中的组织特异性分布和类黄酮生物合成的独特转录调控。CsMYB60通过结合CsFLS和CsLAR的启动子激活CsFLS和CsLAR，直接或间接促进CsMYC1、CsWD40和CsTATA-box结合蛋白的表达，使这四种蛋白形成复合物，增加Cs4CL的表达，并与CsTATA-box结合蛋白相互作用，调节CsCHS的表达，从而调控黄瓜黄酮醇和PAs的生物合成。本研究为揭示类黄酮生物合成的分子机制提供了新的思路，为提高黄瓜果实品质的分子育种提供了依据。
Flavonols and proanthocyanidins (PAs) are the main pigments in the black spines of cucumber (Cucumis sativus) fruit, and CsMYB60 is a key regulator of the biosynthesis of flavonols and PAs. However, in cucumber, the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by CsMYB60 remain unclear. In this study, we clarified the tissue-specific distribution of flavonoids and the unique transcriptional regulation of flavonoid biosynthesis in cucumber. CsMYB60 activated CsFLS and CsLAR by binding to their promoters and directly or indirectly promoted the expression of CsbHLH42, CsMYC1, CsWD40, and CsTATA-box binding protein, resulting in the formation of complexes of these four proteins to increase the expression of Cs4CL and interact with CsTATA-box binding protein to regulate the expression of CsCHS, thereby regulating the biosynthesis of flavonols and PAs in cucumber. Our data provide new insights into the molecular mechanism of flavonoid biosynthesis, which will facilitate molecular breeding to improve fruit quality in cucumber.