T-box gene eomesodermin and the homeobox-containing mix/bix gene mtx2 regulate epiboly movements in the zebrafish
T-box gene eomesodermin and the homeobox-containing mix/bix gene mtx2 regulate epiboly movements in the zebrafish
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DOI：
10.1002/dvdy.20305
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发表时间：
2005-05-01
影响因子：
2.5
通讯作者：
Ho, RK
中科院分区：
文献类型：
--
作者：
Bruce, AEE;Howley, C;Ho, RK
T-box基因eomesodermin（eomes）与斑马鱼和青蛙的中胚层特化和模式化有关。在这里，我们描述了一个额外的功能，为eomes在控制形态发生。上皮细胞片的扩展和变薄，是许多物种原肠胚形成的核心组成部分;然而，尽管其重要性，对其分子控制知之甚少。在这里，我们表明，在斑马鱼胚胎中的eomes功能的抑制显着抑制epiboly运动。我们还表明，eomes调控合子同源异型盒转录因子mtx 2的表达。同样，使用反义吗啉代寡核苷酸敲除mtx 2基因，导致抑制epiboly;此外，我们表明，敲除mtx 2功能的胚外卵黄合胞体层仅足以导致epiboly缺陷。因此，我们已经确定了两个组件的分子途径控制epiboly和显示，深层细胞的胚胎适当的和胚外组织之间的相互作用，有助于在一个协调的方式不同方面的epiboly运动。(c)2005 Wiley-Liss，Inc.
The T-box gene eomesodermin (eomes) has been implicated in mesoderm specification and patterning in both zebrafish and frog. Here, we describe an additional function for eomes in the control of morphogenesis. Epiboly, the spreading and thinning of an epithelial cell sheet, is a central component of gastrulation in many species; however, despite its importance, little is known about its molecular control. Here, we show that repression of eomes function in the zebrafish embryo dramatically inhibits epiboly movements. We also show that eomes regulates the expression of a zygotic homeobox transcription factor mtx2. Gene knockdown of mtx2 using antisense morpholino oligonucleotides, likewise, leads to an inhibition of epiboly; moreover, we show that knockdown of mtx2 function in the extraembryonic yolk syncytial layer only is sufficient to cause epiboly defects. Thus, we have identified two components in a molecular pathway controlling epiboly and show that interactions between deep layer cells of the embryo proper and extraembryonic tissues contribute in a coordinated manner to different aspects of epiboly movements. (c) 2005 Wiley-Liss, Inc.