The temporal relationship between glomerular cell proliferation and monocyte infiltration in Experimental glomerulonephritis
The temporal relationship between glomerular cell proliferation and monocyte infiltration in Experimental glomerulonephritis
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实验性肾小球肾炎肾小球细胞增殖与单核细胞浸润的时间关系
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
M. Perfetto
中科院分区：
文献类型：
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作者：
R. Sterzel;R. Pabst;M. Kregeler;M. Perfetto
在大鼠抗肾小球基底膜肾炎的加速型过程中，作者确定了（a）肾小球细胞过多，（B）细胞增殖动力学和（c）肾小球中单核细胞浸润之间的时间关系。虽然肾小球细胞过多是不明显的或轻度的定性组织学，平均肾小球细胞计数上升之间的第二天和第六天的肾炎高达30%以上的控制。用3 H-胸腺嘧啶核苷脉冲标记后2 h，通过测定组织放射自显影细胞标记指数（LI）和测量离体肾小球的总放射性（TGA）来评估肾小球中的细胞增殖。在第一天，Bowman囊中细胞的平均LI比对照增加356%，随后下降。毛丛细胞LI值的上升更为缓慢，在第4天达到405%的最大增量。TGA也在第一天和第二天增加，并在第四天达到峰值。在其他大鼠中，平行研究，3 H-胸苷掺入测量脉冲后48小时，以允许时间的标记和肾小球内流的单核细胞来自分裂肾外前体。在肾炎的第一天处死的大鼠中，延长标记时间并没有导致进一步获得的簇LI或TGA超过脉冲后2小时获得的值。相反，在第四天，48小时标记时间与LI和TGA结果显著高于2小时间隔相关。这些结果表明，标记的血源性单核细胞的肾小球流入量在第一天是不明显的，但在第二天和第四天之间显着增加。非特异性酯酶的组织化学研究支持了这一点，该研究揭示了肾小球中单核细胞存在的类似时间过程，显示早期阶段很少有酯酶阳性单核细胞，第二天后有许多。结果表明，该模型的肾小球肾炎的特征在于显着增强的复制的内在肾小球细胞，在损伤的第一天开始的时候，当肾小球流入的单核细胞是不明显的。肾小球细胞增殖的增加在随后的几天内变得最明显，当它们与相当大的单核细胞浸润相关时。在没有组织学上令人印象深刻的肾小球细胞过多的情况下，应用定量方法显示肾小球中显著的细胞反应。
SummaryDuring the course of an accelerated form of anti-glomerular basement membrane nephritis in rats, the authors determined the temporal relationships between (a) glomerular hypercellularity, (b) kinetics of cell proliferation and (c) monocyte infiltration in glomeruli. Although glomerular hypercellularity was not appreciable or mild by qualitative histology, mean glomerular cell counts rose between days two and six of nephritis by up to 30% over controls. Cell proliferation in glomeruli was assessed 2 h after pulse label with3H-thymidine by determining histoautoradiographic cell labeling indices (LI) and by measuring the total radioactivity of isolated glomeruli (TGA). On day one, mean LI of cells in Bowman’s capsule was increased by 356% over controls and fell subsequently. LI values of tuft cells rose more gradually, reaching a maximum increment of 405% on day four. TGA also increased on day one and two, and peaked on day four. In additional rats, studied in parallel,3H-thymidine incorporation was measured 48 h after the pulse in order to allow time for the labeling and glomerular influx of monocytes derived from dividing extra-renal precursors. In rats sacrificed on the first day of nephritis, the prolonged labeling time did not result in further gains of tuft LI or of TGA over values obtained 2 h after the pulse. On day four, by contrast, the 48-h labeling time was associated with markedly higher results for LI and TGA than the 2-h interval. These findings indicated that the glomerular influx of labeled blood-borne monocytes was not appreciable on day one but increased markedly between day two and day four. This was supported by histochemical studies for non-specific esterase which revealed a similar time course for the presence of monocytes in glomeruli by showing few esterase-positive monocytes in the early stage and many after day two. The results demonstrate that this model of glomerulonephritis is characterized by markedly enhanced replication of intrinsic glomerular cells, beginning on the first day of the injury at a time when the glomerular influx of monocytes is inconspicuous. Increases of glomerular cell prolifertion become most pronounced over the following days when they are associated with a sizeable monocytic infiltrate. The prominent cellular response in glomeruli is revealed by the applied quantitative methods in the absence of histologically impressive glomerular hypercellularity.