Effect of two-step functionalization of Ti by chemical processes on protein adsorption
Effect of two-step functionalization of Ti by chemical processes on protein adsorption
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DOI：
10.1016/j.apsusc.2011.03.120
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发表时间：
2011-07-15
影响因子：
6.7
通讯作者：
Janik-Czachor, M.
中科院分区：
文献类型：
--
作者：
Pisarek, M.;Roguska, A.;Janik-Czachor, M.
钛及其合金因其优越的机械性能、低模量、优异的耐腐蚀性和良好的生物相容性而广泛用于骨科和牙科植入物。然而，钛植入物和骨组织需要几个月的时间才能达到整合。因此，人们对缩短骨整合过程从而减少手术限制越来越感兴趣。人们通过各种表面改性在金属表面形成具有生物活性的氧化钛层，从而加速骨整合。目前的工作表明，通过在氢氧化钠（NaOH）或过氧化氢/磷酸（H3PO4/H2O2，体积比为1:1）溶液中蚀刻在钛基底上形成二氧化钛（TiO2）层是非常适合类磷灰石涂层沉积的预处理。采用两步程序（在碱性或酸性溶液中蚀刻，然后浸泡在汉克斯介质中），仿生磷酸钙涂层沉积在多孔 TiO2 层上。介绍了表面形貌和化学对磷酸钙层形成的综合影响。使用 HR-SEM 和 AFM 技术对 TiO2 层的形貌进行了表征。使用 HR-SEM 显微镜研究了汉克斯溶液中沉积在 TiO2 多孔层上的磷酸钙 (Ca-P) 涂层的成核和生长。使用 AES、XPS 和 FTIR 表面分析技术对磷酸钙涂层沉积前后以及蛋白质吸附过程后的二氧化钛层进行表征。为了评估此类材料在生物医学应用中的潜在用途，研究了血清白蛋白（血液中最丰富的蛋白质）在此类表面上的吸附。 (C) 2011 Elsevier B.V. 保留所有权利。
Titanium and its alloys are widely used for orthopedic and dental implants because of their superior mechanical properties, low modulus, excellent corrosion resistance and good biocompatibility. However, it takes several months for titanium implants and bone tissue to reach integration. Hence, there is growing interest in shortening the process of osseointegration and thereby reducing surgical restrictions. Various surface modifications have been applied to form a bioactive titanium oxide layer on the metal surface, which is known to accelerate osseointegration.The present work shows that titanium dioxide (TiO2) layers formed on titanium substrates by etching in a solution of sodium hydroxide (NaOH) or hydrogen peroxide/phosphoric acid (H3PO4/H2O2, with a volume ratio of 1:1) are highly suitable pre-treatments for apatite-like coating deposition. Using a two-step procedure (etching in an alkaline or acidic solution followed by soaking in Hanks' medium), biomimetic calcium phosphate coatings were deposited on porous TiO2 layers. The combined effects of surface topography and chemistry on the formation of the calcium phosphate layer are presented. The topography of the TiO2 layers was characterized using HR-SEM and AFM techniques. The nucleation and growth of calcium phosphate (Ca-P) coatings deposited on TiO2 porous layers from Hanks' solution was investigated using HR-SEM microscopy. AES, XPS and FTIR surface analytical techniques were used to characterize the titanium dioxide layers before and after deposition of the calcium phosphate coatings, as well as after the process of protein adsorption. To evaluate the potential use of such materials for biomedical applications, the adsorption of serum albumin, the most abundant protein in the blood, was studied on such surfaces. (C) 2011 Elsevier B. V. All rights reserved.