Antioxidant and Anti-Inflammatory Properties of Anthocyanins Extracted from Oryza sativa L. in Primary Dermal Fibroblasts
Antioxidant and Anti-Inflammatory Properties of Anthocyanins Extracted from Oryza sativa L. in Primary Dermal Fibroblasts
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DOI：
10.1155/2019/2089817
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发表时间：
2019-07-31
影响因子：
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通讯作者：
Nararatwanchai，Thamthiwat
中科院分区：
文献类型：
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作者：
Palungwachira，Pakhawadee;Tancharoen，Salunya;Nararatwanchai，Thamthiwat
风味物质是天然活性物质，形成大量的酚类化合物，在某些食物中丰富。黑米（Oryza sativa L.）含有高含量的花青素多酚，由于其抗氧化特性，对健康有益。随着老化或皮肤退化，胶原网络的破坏导致皮肤伤口愈合受损。因此，恢复停滞的胶原蛋白合成可以帮助维持伤口愈合期间的真皮稳态。本研究对从水稻（Oryza sativa L.）中提取的花青素（ANT）的细胞活性进行了评价，为开发支持自然愈合过程的新产品提供必要的信息。水稻ANT的相对组成。通过高效液相色谱/二极管阵列检测器测定。ANT促进大鼠皮肤成纤维细胞（RDFs）的迁移，并表现出抗氧化特性。ANT增加H2 O2刺激的RDFs中I型胶原α 2（COL 1A 2）的mRNA表达，并上调I型胶原蛋白水平，而无细胞毒性。与未处理组相比，在H2 O2存在下用ANT处理RDFs导致信号通路的激活，包括细胞外信号调节蛋白激酶1和2（ERK 1/2）和Akt，而在H2 O2存在下用ANT处理RDFs显著降低了细胞外信号调节蛋白激酶1和2（ERK 1/2）和Akt。（p< 0.001）抑制IκBα的磷酸化并抑制核因子-κ B（NF-κB）亚单位p50和p65的活化，它们是负责炎症的转录因子。综上所述，我们的研究结果表明，ANT从Oryza sativa L.通过调节I型胶原基因表达和抑制皮肤成纤维细胞中H2 O2诱导的NF-κB活化，具有抗炎特性和抗衰老潜力。
Flavonoids are naturally active substances that form a large class of phenolic compounds abundant in certain foods. Black rice (Oryza sativaL.) contains high levels of anthocyanin polyphenols, which have beneficial effects on health owing to their antioxidant properties. The breakdown of collagenous networks with aging or skin deterioration results in the impairment of wound healing in the skin. Accordingly, reviving stagnant collagen synthesis can help maintain dermal homeostasis during wound healing. This study presents an assessment of the cellular activity of anthocyanins (ANT) extracted fromOryza sativaL., providing information necessary for the development of new products that support natural healing processes. The relative composition of ANT fromOryza sativaL. was determined by high‐performance liquid chromatography/diode array detection. ANT promoted the migration of rat dermal fibroblasts (RDFs) and demonstrated antioxidant properties. ANT increased the mRNA expression ofcollagen type I alpha 2(COL1A2) and upregulated type I collagen protein levels in H2O2‐stimulated RDFs without cytotoxicity. Compared with the untreated group, treatment of RDFs with ANT in the presence of H2O2led to the activation of signaling pathways, including the extracellular signal‐regulated protein kinases 1 and 2 (ERK1/2) and Akt, whereas it significantly (p< 0.001) inhibited the phosphorylation of IκBαand suppressed the activation of the nuclear factor‐kappa B (NF‐κB) subunits, p50 and p65, which are transcription factors responsible for inflammation. Taken together, our findings suggest that ANT fromOryza sativaL. have anti‐inflammatory properties and antiaging potential by modulating type I collagen gene expression and suppressing H2O2‐induced NF‐κB activation in skin fibroblasts.