DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions
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DOI：
10.1093/nar/gkaa600
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发表时间：
2020-09-18
影响因子：
14.9
通讯作者：
Kosuri, Sriram
中科院分区：
文献类型：
--
作者：
Sidore, Angus M.;Plesa, Calin;Kosuri, Sriram
Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a lowcost, multiplexed method that builds gene libraries by compartmentalizing and assembling microarray-derived oligonucleotides in vortexed emulsions. By optimizing enzyme choice, adding enzymatic error correction and increasing scale, we show that Drop-Synth can build thousands of gene-length fragments at >20% fidelity.
