Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans
Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans
复制标题
mRNA输出因子Sus1在白色念珠菌氧化应激耐受中的作用
DOI：
10.1016/j.bbrc.2018.01.044
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发表时间：
2018
影响因子：
3.1
通讯作者：
Mingchun Li
中科院分区：
文献类型：
--
作者：
Chenpeng Xiao;Qilin Yu;Bing Zhang;Jianrong Li;Dan Zhang;Mingchun Li
在真核生物中，mRNA的核输出对于基因表达至关重要。然而，很少有人知道的重要真菌病原体，白色念珠菌的mRNA核输出的作用。在这项研究中，我们鉴定了白色念珠菌Sus 1，一种mRNA输出所需的核定位蛋白。有趣的是，thesus 1 Δ/Δ表现出对细胞外氧化应激的超敏感性，增强ROS积累和严重的氧化应激相关的细胞死亡。更引人注目的是，虽然该突变体表现出正常的氧化应激反应（OSR）基因的表达激活，它具有减弱活性的ROS清除系统，这可能是由于在该突变体中的缺陷OSR mRNA输出。此外，菌株1 Δ/Δ的毒力严重减弱。总之，我们的研究结果提供了证据表明，mRNA输出因子Sus 1在氧化应激耐受和发病机制中起着重要作用。
In eukaryotes, the nuclear export of mRNAs is essential for gene expression. However, little is known about the role of mRNA nuclear export in the important fungal pathogen,Candida albicans. In this study, we identifiedC. albicansSus1, a nucleus-localized protein that is required for mRNA export. Interestingly, thesus1Δ/Δ displayed hyper-sensitivity to extracellular oxidative stress, enhanced ROS accumulation and severe oxidative stress-related cell death. More strikingly, although the mutant exhibited normal activation of the expression of oxidative stress response (OSR) genes, it had attenuated activity of ROS scavenging system, which may be attributed to the defect in OSR mRNA export in this mutant. In addition, the virulence of thesus1Δ/Δ was seriously attenuated. Taken together, our findings provide evidence that the mRNA export factor Sus1 plays an important role in oxidative stress tolerance and pathogenesis.