Antifouling Activity towards Mussel by Small-Molecule Compounds from a Strain of Vibrio alginolyticus Bacterium Associated with Sea Anemone Haliplanella sp.
Antifouling Activity towards Mussel by Small-Molecule Compounds from a Strain of Vibrio alginolyticus Bacterium Associated with Sea Anemone Haliplanella sp.
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DOI：
10.4014/jmb.1607.07068
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发表时间：
2017-03
影响因子：
2.8
通讯作者：
Xiang Wang;Yanqiu Huang;Y. Sheng;Pei Su;Y. Qiu;C. Ke;Danqing Feng
中科院分区：
文献类型：
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作者：
Xiang Wang;Yanqiu Huang;Y. Sheng;Pei Su;Y. Qiu;C. Ke;Danqing Feng
贻贝是造成严重技术和经济问题的主要污损生物。在这项研究中，对贻贝的抗菌活性被发现在三个化合物中分离出的海洋细菌与海葵Haliplanella sp.这种细菌菌株，称为PE 2，被确定为溶藻弧菌使用形态学，生化测试，和系统发育分析的基础上的序列的16 S rRNA和四个管家基因（rpoD，gyrB，rctB，和toxR）。利用柱层析技术从溶藻弧菌PE 2的乙酸乙酯提取物中纯化了三种小分子化合物（吲哚、3-甲酰吲哚和环（Pro-Leu））。吲哚、3-甲酰基吲哚和环（脯氨酸）亮氨酸对绿色贻贝（佩尔纳viridis）的EC_（50）分别为24.45 μg/ml、50.07 μg/ml和49.24 μg/ml。海洋细菌代谢产物对贻贝的促生长活性研究较少。吲哚、3-甲酰吲哚和环（Pro-Leu）对藤壶白脊藤壶（Balanus albicostatus）和海洋细菌假单胞菌（Pseudomonas sp.）的沉降也表现出抑制活性（EC 50值分别为8.84、0.43和11.35 μg/ml），EC 50值分别为42.68、69.68和39.05 μg/ml）。这些结果表明，这三种化合物对于环境友好的贻贝控制和/或有效对抗几种生物污损物的新的生物污损物添加剂的开发是潜在有用的。
Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp. This bacterial strain, called PE2, was identified as Vibrio alginolyticus using morphology, biochemical tests, and phylogenetic analysis based on sequences of 16S rRNA and four housekeeping genes (rpoD, gyrB, rctB, and toxR). Three small-molecule compounds (indole, 3-formylindole, and cyclo (Pro-Leu)) were purified from the ethyl acetate extract of V. alginolyticus PE2 using column chromatography techniques. They all significantly inhibited byssal thread production of the green mussel Perna viridis, with EC50 values of 24.45 μg/ml for indole, 50.07 μg/ml for 3-formylindole, and 49.24 μg/ml for cyclo (Pro-Leu). Previous research on the antifouling activity of metabolites from marine bacteria towards mussels is scarce. Indole, 3-formylindole and cyclo (Pro-Leu) also exhibited antifouling activity against settlement of the barnacle Balanus albicostatus (EC50 values of 8.84, 0.43, and 11.35 μg/ml, respectively) and the marine bacterium Pseudomonas sp. (EC50 values of 42.68, 69.68, and 39.05 μg/ml, respectively). These results suggested that the three compounds are potentially useful for environmentally friendly mussel control and/or the development of new antifouling additives that are effective against several biofoulers.