Suppressive Effects of the Leaf of Terminalia catappa L. on Osteoclast Differentiation In Vitro and Bone Weight Loss In Vivo
Suppressive Effects of the Leaf of Terminalia catappa L. on Osteoclast Differentiation In Vitro and Bone Weight Loss In Vivo
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DOI：
10.3177/jnsv.58.129
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发表时间：
2012-04-01
影响因子：
1.6
通讯作者：
Yazawa, Kazunaga
中科院分区：
文献类型：
--
作者：
Koyama, Tomoyuki;Nakajima, Chie;Yazawa, Kazunaga
昆布提取物(TCE；1,000 mg/kg)灌胃5wk可抑制去卵巢小鼠的骨量丢失和骨小梁丢失。体外实验表明，TCE(1.3-20mUg/mL)不能增加成骨细胞样3T3-L1细胞碱性磷酸酶的活性，表明破骨细胞的形成。另一方面，在骨髓细胞/成骨细胞UAMS-32共培养体系中，TCE(12.5mUg/mLTCE)显著减少抗酒石酸酸性磷酸酶(TRAP)阳性的多核细胞的数量，这可能表明破骨细胞的形成。对破骨细胞分化阶段的详细分析表明，在M-CSF和转化生长因子-β的存在下，TCE主要抑制骨髓单个核细胞向破骨细胞前体细胞的分化。另一项使用分级三氯乙烯的实验表明，水溶性部分抑制了去卵巢小鼠的骨重量损失和体外破骨细胞分化。因此，三氯乙烯对小鼠骨质量下降的抑制作用可能与高极性成分对破骨细胞分化早期的抑制作用有关。
Oral administration of Terminalia catappa extract (TCE; 1,000 mg/kg) for 5 wk suppressed bone weight loss and trabecular bone loss in ovariectomized mice. An in vitro experiment showed that TCE (1.3-20 mu g/mL) did not increase alkaline phosphatase activity, which would indicate osteoclast formation, in osteoblast-like 3T3-L1 cells. On the other hand, TCE (12.5 mu g/mL) markedly decreased the number of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells, which would indicate osteoclast formation, in a co-culture system (bone marrow cells/osteoblastic UAMS-32 cells). A detailed analysis of the stages of osteoclast differentiation revealed that TCE mainly suppressed the differentiation of bone marrow mononuclear cells into osteoclast progenitor cells in the presence of M-CSF and TGF-beta. An additional experiment using fractionated TCE revealed that the water-soluble fraction suppressed the bone weight loss in OVX-mice and osteoclast differentiation in vitro. Therefore, the suppressive effects of TCE on bone weight loss in mice might be due to the suppressive effects of highly polar components on the early stage of osteoclast differentiation.