Varicella-zoster virus (VZV) ORF65 virion protein is dispensable for replication in cell culture and is phosphorylated by casein kinase II, but not by the VZV protein kinases
Varicella-zoster virus (VZV) ORF65 virion protein is dispensable for replication in cell culture and is phosphorylated by casein kinase II, but not by the VZV protein kinases
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DOI：
10.1006/viro.2000.0741
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发表时间：
2001-02-01
期刊：
影响因子：
3.7
通讯作者：
Lekstrom, K
中科院分区：
文献类型：
--
作者：
Cohen, JI;Sato, H;Lekstrom, K
水痘检测病毒(VZV)独特的短区编码4个基因。其中一个，ORF65，被预测编码11 kDa的蛋白质。抗ORF65蛋白的抗体从VZV感染细胞膜部分免疫沉淀出一种16 kDa的蛋白。ORF65蛋白被酪蛋白激酶II磷酸化，而VN、ORF47和ORF66不是ORF65蛋白磷酸化所必需的。构建了ORF65大片段缺失的VN，证明VN对于细胞培养中病毒的复制是必不可少的。VZV ORF65的单纯疱疹病毒同源基因已被报道定位于感染呼唤的细胞核和病毒粒子中，而伪狂犬病病毒的同源基因则以II型膜蛋白的形式存在于感染细胞的高尔基体和病毒粒子中。ORF65蛋白定位于病毒感染细胞中的高尔基体，定位于病毒粒子中，很可能是一种II型膜蛋白。因此，VN ORF65在感染细胞和病毒粒子中的定位更接近于其伪狂犬病病毒同源物。
The unique short region of varicella tester virus (VZV) encodes four genes. One of these, ORF65, is predicted to encode an 11-kDa protein. Antibody to ORF65 protein immunoprecipitated a 16-kDa protein from the membrane fraction of VZV-infected cells. ORF65 protein was shown to be phosphorylated by casein kinase II. The VN ORF47 or ORF66 protein kinases were not required for phosphorylation of ORF65. VN with a large deletion in ORF65 was constructed and was shown to be dispensable for replication of virus in cell culture. The herpes simplex virus homolog of VZV ORF65 has been reported to be located in the nucleus of infected calls and in virions as a tegument protein, whereas the pseudorabies virus homolog is located in the Golgi apparatus of infected cells and in virions as a type II membrane protein. The ORF65 protein localized to the Golgi apparatus in virus-infected cells and was located in virions, most likely as a type II membrane protein. Thus, VN ORF65 more closely resembles its pseudorabies virus homolog in its localization in infected cells and virions.