Brain creatine kinase and creatine transporter proteins in normal and creatine-treated rabbit pups.
Brain creatine kinase and creatine transporter proteins in normal and creatine-treated rabbit pups.
复制标题
正常和肌酸处理的幼兔的脑肌酸激酶和肌酸转运蛋白。
DOI：
10.1159/000017473
复制
发表时间：
2000
影响因子：
2.9
通讯作者：
Holtzman,D
中科院分区：
文献类型：
--
作者：
Kekelidze,T;Khait,I;Togliatti,A;Holtzman,D
全身补充肌酸（Cr）增加脑磷酸肌酸（PCr），并防止15日龄兔缺氧性癫痫发作。在正常发育的5 - 30日龄之间，兔灰质线粒体肌酸激酶（Mi-CK）增加400%，而胞质CK（BB-CK）增加60%。在白色物质中，这两种同工酶在此期间表现出较小的相似增加（40%）。在这两个地区的铬转运蛋白减少60%之间的5和15天。在活体条件下，用~（31）P核磁共振法测得的以灰质为主的脑片的CK速率常数在10 ~ 20天增加30%，20 ~ 30天下降50%。在以白色物质为主的切片中也可以看到类似的成熟变化。注射铬在15天没有显着改变BB-CK或Mi-CK同工酶或在体内CK反应速率常数。因此，在CK系统与抑制缺氧性癫痫发作在铬处理的兔子的最大变化是增加PCr的灰色和白色的问题。
Systemic creatine (Cr) supplementation increases brain phosphocreatine (PCr) and prevents hypoxic seizures in 15-day-old rabbits . Between 5 and 30 days of age during normal development, rabbit gray matter mitochondrial creatine kinase (Mi-CK) increases 400% while cytosolic CK (BB-CK) increases 60%. In white matter, both isoenzymes show smaller, similar increases (40%) during this period. The Cr transporter protein decreases 60% between 5 and 15 days in both regions. In vivo CK rate constants measured by31P nuclear magnetic resonance increase 30% between 10 and 20 days, and then fall 50% between 20 and 30 days in predominantly gray matter slices. Similar maturational changes are seen in predominantly white matter slices. Injecting Cr at 15 days does not significantly change BB-CK or Mi-CK isoenzymes or the in vivo CK reaction rate constants. Thus, the largest change in the CK system associated with suppression of hypoxic seizures in Cr-treated rabbits is increased PCr in gray and white matter.