Effect of Annurca Apple Polyphenols on Human HaCaT Keratinocytes Proliferation
Effect of Annurca Apple Polyphenols on Human HaCaT Keratinocytes Proliferation
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DOI：
10.1089/jmf.2012.0076
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发表时间：
2012-11-01
影响因子：
2.4
通讯作者：
Boccellino, Mariarosaria
中科院分区：
文献类型：
--
作者：
D'Angelo, Stefania;La Porta, Raffaele;Boccellino, Mariarosaria
多酚类化合物在体外具有明显的抗氧化活性。然而，在复杂的生物系统中，它们表现出额外的特性，这些特性还知之甚少。苹果是世界上消费量最大的水果之一，一些研究表明，苹果多酚可以在预防退行性疾病方面发挥作用。本研究旨在评估Annurca苹果多酚提取物（APE）对HaCaT细胞增殖和凋亡的影响。这些数据表明，苹果多酚化合物对HaCaT细胞具有显着的抗增殖作用。荧光激活细胞分选分析显示APE以剂量依赖的方式诱导细胞凋亡。此外，苹果多酚通过触发死亡受体相关的非p53依赖的外源性途径诱导上皮细胞凋亡。APE也能够诱导形态学变化，如核浓缩所证明的。细胞，形态学和分子数据明确表明，诱导细胞凋亡是主要负责先前观察到的抗增殖诱导的APE对HaCaT角质形成细胞。我们的实验结果表明，苹果多酚是一个有前途的来源，从天然为基础的局部制剂可以开发用于皮肤疾病的治疗。
Polyphenols have been demonstrated to have clear antioxidant activities in vitro. However, in complex biological systems, they exhibit additional properties, which are yet poorly understood. The apple is among the most consumed fruits worldwide, and several studies suggest that apple polyphenols could play a role in the prevention of degenerative diseases. The present study aimed at evaluating the Annurca apple polyphenol extract (APE) effects both proliferation and apoptosis on HaCaT cells. The data indicate that apple polyphenolic compounds had significant antiproliferative action on HaCaT cells. The fluorescence-activated cell-sorting analysis showed that APE induced cell apoptosis in a dose-dependent manner. Moreover, apple polyphenols induced apoptosis in epithelial cells by triggering a death receptor-associated extrinsic pathway p53-independent. APE was also capable of inducing morphological changes as evidenced by nuclear condensation. The cellular, morphological, and molecular data unequivocally demonstrated that induction of cellular apoptosis was mainly responsible for the previously observed antiproliferation-induced APE on HaCaT keratinocytes. Our experimental results suggest that apple polyphenols are a promising source from which a natural-based topical agent could be developed for skin diseases treatment.