Validation of High-Resolution Tractography Against In Vivo Tracing in the Macaque Visual Cortex.
Validation of High-Resolution Tractography Against In Vivo Tracing in the Macaque Visual Cortex.
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DOI：
10.1093/cercor/bhu326
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发表时间：
2015-11
期刊：
影响因子：
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通讯作者：
Parker GJ
中科院分区：
文献类型：
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作者：
Azadbakht H;Parkes LM;Haroon HA;Augath M;Logothetis NK;de Crespigny A;D'Arceuil HE;Parker GJ
扩散磁共振成像 (MRI) 可以对人脑的解剖连接进行无创体内检查，这对于了解大脑功能具有重要作用。该技术的验证至关重要，但由于缺乏足够的金标准，验证起来很困难。在这项工作中，猕猴视觉系统被用作模型，因为体内和死后示踪剂研究的大量文献已经建立了对潜在联系的详细理解。我们对 2 个离体、体外猕猴大脑的高角分辨率扩散成像数据进行了概率纤维束成像。对不同概率连接“强度”阈值下识别的连接、文献中先前提出的各种跟踪优化策略以及 Felleman 和 Van Essen (1991; FVE91) 描述的详细视觉系统接线图中的已知连接进行了比较。平均而言，通过执行最成功优化的概率扩散 MRI 纤维束成像，可以重现 FVE91 识别出的 74% 的连接。与最近的示踪剂研究结果的进一步比较表明，纤维束成像在估计区域间连接是否存在时的保真度可能比这更高。
Diffusion magnetic resonance imaging (MRI) allows for the noninvasive in vivo examination of anatomical connections in the human brain, which has an important role in understanding brain function. Validation of this technique is vital, but has proved difficult due to the lack of an adequate gold standard. In this work, the macaque visual system was used as a model as an extensive body of literature of in vivo and postmortem tracer studies has established a detailed understanding of the underlying connections. We performed probabilistic tractography on high angular resolution diffusion imaging data of 2 ex vivo, in vitro macaque brains. Comparisons were made between identified connections at different thresholds of probabilistic connection “strength,” and with various tracking optimization strategies previously proposed in the literature, and known connections from the detailed visual system wiring map described by Felleman and Van Essen (1991; FVE91). On average, 74% of connections that were identified by FVE91 were reproduced by performing the most successfully optimized probabilistic diffusion MRI tractography. Further comparison with the results of a more recent tracer study ( ) suggests that the fidelity of tractography in estimating the presence or absence of interareal connections may be greater than this.