De novo expression of MECA-79 glycoprotein-determinant on developing B lymphocytes in gut-associated lymphoid tissues.
De novo expression of MECA-79 glycoprotein-determinant on developing B lymphocytes in gut-associated lymphoid tissues.
复制标题
MECA-79 糖蛋白决定子对肠道相关淋巴组织中发育中的 B 淋巴细胞的从头表达。
DOI：
10.1111/j.1365-2567.2006.02457.x
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发表时间：
2006
期刊：
影响因子：
6.4
通讯作者：
Mage,RoseG
中科院分区：
文献类型：
--
作者：
Sinha,RajeshK;Yang,Guibin;Alexander,Cornelius;Mage,RoseG
Rabbit is one of several species that depend on development of B lymphocytes in gut‐associated lymphoid tissues for primary immunoglobulin‐repertoire diversification. The rabbit appendix is an important site of early B‐lymphocyte development. We previously reported that peripheral lymph node addressin detected by monoclonal antibody (mAb) MECA‐79 played a role in recruitment of immature blood‐borne B cells into neonatal rabbit appendix. Here, we report expression of an ∼127 000 MWO‐linked sulphated proteoglycan on developing B cells in appendix and Peyer's patches recognized by the mAb MECA‐79. Binding of the mAb to B lymphocytes was sensitive to enzyme treatment withO‐sialoglycoprotease and expression was partially inhibited by sodium chlorate, a metabolic inhibitor of sulphation. The proportions of MECA‐79+B lymphocytes gradually increased from < 0·5% at 3 days to > 70% at 6 weeks in appendix and Peyer's patches. The proportions of MECA‐79+B lymphocytes in spleen and peripheral blood were very low (0·5–2%). However, the MECA‐79 determinant was detected on B cells in splenic germinal centres after immunization.In situlabelling of appendix cells showed that the MECA‐79 determinant was expressed on fluorescein‐labelled B lymphocytes that migrated from appendix into mesenteric lymph nodes. B‐cell MECA‐79 may be involved in interactions with T cells and/or dendritic cells. Alternatively, because we found that lymphatic endothelium in the thymus‐dependent area of appendix, a site for lymphocyte exit, expressed P‐selectin (CD62P), interaction of the MECA‐79 determinant on B cells with CD62P may have a role in the exit of B lymphocytes from rabbit appendix.
