IMMUNOGENICITY AND INFECTIVITY OF SPOROZOITES OF MAMMALIAN MALARIA ISOLATED BY DENSITY-GRADIENT CENTRIFUGATION
IMMUNOGENICITY AND INFECTIVITY OF SPOROZOITES OF MAMMALIAN MALARIA ISOLATED BY DENSITY-GRADIENT CENTRIFUGATION
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DOI：
10.1111/j.1550-7408.1973.tb03594.x
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
NUSSENZWEIG, RS
中科院分区：
文献类型：
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作者：
KRETTLI, A;CHEN, DH;NUSSENZWEIG, RS
Sporozoites of rodent and simian malaria (Plasmodium bergheiandP. cynomolgi) were purified by centrifugation on a linear Renografin/BSA gradient. This procedure made it possible to process rapidly a large number of infected mosquitoes leading to the recovery of a considerable proportion of sporozoites. Gradient‐recovered sporozoites (GRS) freed of most bacteria and mosquito tissue contaminants, retained their infectivity and immunogenicity. Mice repeatedly injected i.v. with irradiated GRS ofP. bergheiacquired total protection against an otherwise lethal sporozoite challenge. GRS ofP. bergheiandP. cynomolgiinduced antisporozoite (CSP) antibody production in rats.
