Expression analyses of human endogenous retroviruses (HERVs): tissue-specific and developmental stage-dependent expression of HERVs
Expression analyses of human endogenous retroviruses (HERVs): tissue-specific and developmental stage-dependent expression of HERVs
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DOI：
10.1016/j.ygeno.2004.09.004
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发表时间：
2004-12-01
期刊：
影响因子：
4.4
通讯作者：
Kanaya, F
中科院分区：
文献类型：
--
作者：
Okahara, G;Matsubara, S;Kanaya, F
与L1相比，人类内源性逆转录病毒(HERV)的进化和生物学作用较少被认识。在本研究中，我们重点研究了HERV在正常组织中的转录活性，发现了五个在正常组织中活跃表达的HERV基因座。除1例外，其余均有组织特异性表达：1例在胃和小肠表达，3例在胎盘中表达。我们随后通过基于TaqMan的RT-PCR检测了三个胎盘特异性HERV以及合胞素和合胞素2的时间表达谱，并观察到三种模式。Syncytin和Herv-Fb在整个妊娠过程中几乎持续表达。随着妊娠的进行，Syncytin-2逐渐减少。相反，Herv-H/F和Herv-K(HML-6)基因座在足月胎盘中的表达显著增加。总体而言，足月胎盘的HERV表达水平存在较大的个体差异。我们的结果表明，HERV的影响可能比目前认为的更多样化。(C)2004 Elsevier Inc.保留所有权利。
The evolutional and biological roles of human endogenous retroviruses (HERVs) are less recognized compared to those of L1. In the present study, we focused on the transcriptional activity of HERVs in normal human tissues and found five HERV loci that are actively expressed in normal tissues. All but one showed tissue specificity of expression: one was expressed in stomach and small intestine and three were in placenta. We subsequently examined by TaqMan-based RT-PCR assays the temporal expression profiles of the three placenta-specific HERVs along with syncytin and syncytin 2 and observed three patterns. Syncytin and HERV-Fb showed almost constant expression through gestations. Syncytin 2 gradually decreased as pregnancy proceeded. In contrast, expression from the HERV-H/F and HERV-K(HML-6) loci increased remarkably in term placentas. Term placentas in general showed larger interindividual differences in HERV expression levels. Our results suggest that HERVs might have more diverse effects than currently thought. (C) 2004 Elsevier Inc. All rights reserved.