Barrier screens: a method to sample blood-fed and host-seeking exophilic mosquitoes
Barrier screens: a method to sample blood-fed and host-seeking exophilic mosquitoes
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DOI：
10.1186/1475-2875-12-49
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发表时间：
2013-02-05
期刊：
影响因子：
3
通讯作者：
Lobo, Neil F.
中科院分区：
文献类型：
--
作者：
Burkot, Thomas R.;Russell, Tanya L.;Lobo, Neil F.
背景：通过户外进食和休息的蚊子来确定人类的血餐比例是具有挑战性的。这在很大程度上是由于很难找到足够的和公正的样本休息，饱食蚊子，使主机血餐来源的识别。这是特别困难的，在西南太平洋国家的印度尼西亚，所罗门群岛和巴布亚新几内亚，厚厚的植被构成的主要栖息地的嗜外蚊子，是主要的疟疾和丝虫病vectors.Methods：屏障屏障屏幕的遮阳布网连接到竹竿之间的村庄和可能的地区，蚊子可能寻求血餐或休息。飞行中的蚊子会被屏障阻挡，暂时停止飞行，然后整晚每隔一小时就可以被吸入捕获。在对该方法进行评价的三个国家，法劳蒂按蚊、班氏按蚊、长吻按蚊、孙代按蚊、迷走按蚊、高知按蚊、环状按蚊、棋盘格按蚊、维氏库蚊、当致倦库蚊和曼森氏蚊在屏障上休息时收集。此外，雌性斑点按蚊和阿蚊以及雄性按蚊。法劳蒂角维什努伊角quinquefasciatus和伊蚊物种也同样captured.Conclusions：建设屏障屏幕作为临时休息场所，蚊子可能会飞的地区是一个非常有效的时间的方法，收集一个公正的代表性样本的充血蚊子确定人类血液指数。
Background: Determining the proportion of blood meals on humans by outdoor-feeding and resting mosquitoes is challenging. This is largely due to the difficulty of finding an adequate and unbiased sample of resting, engorged mosquitoes to enable the identification of host blood meal sources. This is particularly difficult in the south-west Pacific countries of Indonesia, the Solomon Islands and Papua New Guinea where thick vegetation constitutes the primary resting sites for the exophilic mosquitoes that are the primary malaria and filariasis vectors.Methods: Barrier screens of shade-cloth netting attached to bamboo poles were constructed between villages and likely areas where mosquitoes might seek blood meals or rest. Flying mosquitoes, obstructed by the barrier screens, would temporarily stop and could then be captured by aspiration at hourly intervals throughout the night.Results: In the three countries where this method was evaluated, blood-fed females of Anopheles farauti, Anopheles bancroftii, Anopheles longirostris, Anopheles sundaicus, Anopheles vagus, Anopheles kochi, Anopheles annularis, Anopheles tessellatus, Culex vishnui, Culex quinquefasciatus and Mansonia spp were collected while resting on the barrier screens. In addition, female Anopheles punctulatus and Armigeres spp as well as male An. farauti, Cx. vishnui, Cx. quinquefasciatus and Aedes species were similarly captured.Conclusions: Building barrier screens as temporary resting sites in areas where mosquitoes were likely to fly was an extremely time-effective method for collecting an unbiased representative sample of engorged mosquitoes for determining the human blood index.