Mechanical stimulation of tissue repair in the hydraulic bone chamber
Mechanical stimulation of tissue repair in the hydraulic bone chamber
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DOI：
10.1359/jbmr.1997.12.8.1295
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发表时间：
1997-08-01
影响因子：
6.2
通讯作者：
Goldstein, SA
中科院分区：
文献类型：
--
作者：
Guldberg, RE;Caldwell, NJ;Goldstein, SA
利用液压激活的骨室模型来研究骨修复过程中机械适应的细胞和微观结构机制，编织骨小梁和纤维化肉芽组织在植入犬胫骨和股骨干骺端后 8 周填充最初空的骨室。在没有机械刺激的情况下，在 8 至 24 周之间观察到板层骨小梁的主动骨重塑和骨髓元素的重建，在随后的负载研究中，在 8 周不受干扰的骨修复后，在 10 只狗的随机选择的一侧激活液压机制，负载治疗施加间歇性压缩力（18 N，1.0 Hz，1800 周期/天），持续几天到 12 周，体视学分析三维微计算机断层扫描图像显示，相对于未负载的对侧对照，与负载的修复组织微观结构相关的小梁板厚度和连接性有所增加，这些微观结构的改变对应于负载骨组织的表观模量增加了600%以上，负载几天后，I型前胶原免疫阳性的成骨细胞排列的小梁表面的百分比显着增加，为骨基质合成的机械刺激提供了进一步的证据，局部主要组织使用基于数字图像的有限元方法估计与这些适应性变化相关的应变范围约为-2000至+3000μ应变。本研究证明了骨组织和细胞对受控体内机械刺激的敏感性，并确定了骨修复过程中机械适应的微观结构机制。引入液压骨室作为有效的实验模型来研究机械和生物因素对骨修复和再生的影响。
A hydraulically activated bone chamber model was utilized to investigate cellular and microstructural mechanisms of mechanical adaptation during bone repair, Woven trabecular bone and fibrotic granulation tissue filled the initially empty chambers by 8 weeks postimplantation into canine tibial and femoral metaphyses. Without mechanical stimulation, active bone remodeling to lamellar trabecular bone and reconstitution of marrow elements were observed between 8 and 24 weeks, In subsequent loading studies, the hydraulic mechanism was activated on one randomly chosen side of 10 dogs following 8 weeks of undisturbed bone repair, The loading treatment applied an intermittent compressive force (18 N, 1.0 Hz, 1800 cycles/day) for durations of a few days up to 12 weeks, Stereological analysis of three-dimensional microcomputed tomography images revealed an increase in trabecular plate thickness and connectivity associated with the loaded repair tissue microstructure relative to unloaded contralateral controls, These microstructural alterations corresponded to an over 600% increase in the apparent modulus of the loaded bone tissue, A significant increase in the percentage of trabecular surfaces lined by osteoblasts immunopositive for type I procollagen after a few days of loading provided further evidence for mechanical stimulation of bone matrix synthesis, The local principal tissue strains associated with these adaptive changes were estimated to range from approximately -2000 to +3000 mu strain using digital image-based finite element methods, This study demonstrates the sensitivity of bone tissue and cells to a controlled in vivo mechanical stimulus and identifies microstructural mechanisms of mechanical adaptation during bone repair, The hydraulic bone chamber is introduced as an efficient experimental model to study the effects of mechanical and biological factors on bone repair and regeneration.