Diversity of arbuscular mycorrhizal fungi colonising roots of the grass species Agrostis capillaris and Lolium perenne in a field experiment
Diversity of arbuscular mycorrhizal fungi colonising roots of the grass species Agrostis capillaris and Lolium perenne in a field experiment
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DOI：
10.1007/s00572-003-0244-7
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发表时间：
2004-04-01
期刊：
影响因子：
3.9
通讯作者：
Atkinson, D
中科院分区：
文献类型：
--
作者：
Gollotte, A;van Tuinen, D;Atkinson, D
通过孢子和根内菌丝的形态特征分析丛枝菌根（AM）真菌的多样性，以前建议，优先协会之间发生的植物和AM真菌。通过田间试验研究了不同寄主植物对高地草原AM真菌多样性的影响。土著植被地块在未经改善的牧场被替换为单一栽培的剪股颖或多年生黑麦草。修改AM真菌在这些地块的多样性进行了评估大亚基（LSU）核糖体RNA（rDNA）基因的部分序列分析。设计AM真菌通用引物，用AM真菌根组织DNA进行PCR扩增。capillaris和L.多年生植物PCR产物用于构建LSU rDNA文库。随机选择的克隆测序表明，植物根部的AM真菌属于球囊霉属，无梗孢属和盾孢属的殖民。定植于A根部的AM真菌多样性存在差异。capillaris和L.通过使用对每个序列组特异的引物的PCR来确认。这些分子数据表明，植物对AM真菌群落存在选择压力。
Analysis of arbuscular mycorrhizal (AM) fungal diversity through morphological characters of spores and intraradicular hyphae has suggested previously that preferential associations occur between plants and AM fungi. A field experiment was established to investigate whether AM fungal diversity is affected by different host plants in upland grasslands. Indigenous vegetation from plots in an unimproved pasture was replaced with monocultures of either Agrostis capillaris or Lolium perenne. Modification of the diversity of AM fungi in these plots was evaluated by analysis of partial sequences in the large subunit (LSU) ribosomal RNA (rDNA) genes. General primers for AM fungi were designed for the PCR amplification of partial sequences using DNA extracted from root tissues of A. capillaris and L. perenne. PCR products were used to construct LSU rDNA libraries. Sequencing of randomly selected clones indicated that plant roots were colonised by AM fungi belonging to the genera Glomus, Acaulospora and Scutellospora. There was a difference in the diversity of AM fungi colonising roots of A. capillaris and L. perenne that was confirmed by PCR using primers specific for each sequence group. These molecular data suggest the existence of a selection pressure of plants on AM fungal communities.