Scanning electrochemical microscopy of living cells.: 3.: Rhodobacter sphaeroides
Scanning electrochemical microscopy of living cells.: 3.: Rhodobacter sphaeroides
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DOI：
10.1021/ac010945e
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发表时间：
2002-01-01
影响因子：
7.4
通讯作者：
Rusling, JF
中科院分区：
文献类型：
--
作者：
Cai, CX;Liu, B;Rusling, JF
采用扫描电化学显微镜（SECM）对单个紫色细菌（球形红杆菌）的氧化还原活性进行了检测。我们之前在哺乳动物细胞研究中发展的方法被扩展到测量细菌跨膜电荷转移的速率和研究途径。两组氧化还原介质（即亲水性和疏水性氧化还原物质）用于在溶液中的SECM尖端电极和细胞内的氧化还原中心之间穿梭电子。通过分析测量的速率常数与溶液中介质的形式电位和浓度的关系，可以得到有关外细胞膜对不同离子的渗透性和细胞内氧化还原性质的信息。以微米或亚微米的空间分辨率获得细胞表面的氧化还原反应性图。
The scanning electrochemical microscope (SECM) was used to probe the redox activity of individual purple bacteria (Rhodobacter sphaeroides). The approaches developed in our previous studies of mammalian cells were expanded to measure the rates and investigate the pathway of transmembrane charge transfer in bacteria. The two groups of redox mediators (i.e., hydrophilic and hydrophobic redox species) were used to shuttle the electrons between the SECM tip electrode in solution and the redox centers inside the cell. The analysis of the dependencies of the measured rate constant on formal potential and concentration of mediator species in solution yielded information about the permeability of the outer cell membrane to different ionic species and intracellular redox properties. The maps of redox reactivity of the cell surface were obtained with a micrometer or submicrometer spatial resolution.