Inhibition of inducible nitric oxide synthase expression and cell death by (-)-epigallocatechin-3-gallate, a green tea catechin, in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson's disease
Inhibition of inducible nitric oxide synthase expression and cell death by (-)-epigallocatechin-3-gallate, a green tea catechin, in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson's disease
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DOI：
10.1016/j.jocn.2010.01.042
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发表时间：
2010-09-01
影响因子：
2
通讯作者：
Jeon, Beom S.
中科院分区：
文献类型：
--
作者：
Kim, Ji Seon;Kim, Jong-Min;Jeon, Beom S.
本研究的目的是调查诱导型一氧化氮合酶 (iNOS) 在 (-)-表没食子儿茶素-3-没食子酸酯 (EGCG)（一种针对帕金森病 (PD) 的潜在神经保护剂）作用中的参与，并测试高剂量 EGCG 产生的毒性。将 EGCG 以两种不同剂量（10 mg/kg 和 50 mg/kg）给予 1-甲基-4-苯基-1,2,3,6-四氢吡啶 (MPTP) 小鼠。 EGCG治疗将神经元死亡率降低至50%以下。 MPTP组中iNOS表达水平比对照组高20%，但EGCG组中iNOS表达水平降低至阴性对照组中观察到的水平。两种剂量的 EGCG 对于细胞拯救同样有益，并且较高剂量没有观察到毒性。 iNOS 的抑制可能是预防 MPTP 毒性的重要机制，而 EGCG 可能是针对 PD 的潜在神经保护剂。 (C) 2010 Elsevier Ltd. 保留所有权利。
The aim of this study was to investigate the involvement of inducible nitric oxide synthase (iNOS) in the action of (-)-epigallocatechin-3-gallate (EGCG), a potential neuroprotective agent against Parkinson's disease (PD), and to test for toxicity resulting from high doses of EGCG. EGCG was administered to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice at two different doses (10 mg/kg and 50 mg/kg). EGCG treatment reduced the neuronal death rate to less than 50%. The level of iNOS expression in the MPTP group was 20% higher than that seen in the control group, but in the EGCG groups, iNOS expression was reduced to the level observed in the negative control group. The two doses of EGCG were equally beneficial for cell rescue, and no toxicity was observed with the higher dose. Inhibition of iNOS may be an important mechanism underlying the prevention of MPTP toxicity, and EGCG may potentially be a neuroprotective agent against PD. (C) 2010 Elsevier Ltd. All rights reserved.